A reliable general purpose method for extracting genomic DNA from Dictyostelium cells.

A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Research Abstract Details 

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A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Abstract Text:

Karen E Pilcher,Petra Fey,Pascale Gaudet,Anthony S Kowal,Rex L Chisholm,Karen E Pilcher,Petra Fey,Pascale Gaudet,Anthony S Kowal,Rex L Chisholm,

In this protocol, we present a standard method for extracting DNA from cells of the social amoeba Dictyostelium discoideum. While this procedure is similar to other phenol:chloroform-based purification methods, it is modified to account for the high level of carbohydrate and nucleases found in Dictyostelium cells. Genomic DNA can be isolated from wild-type and genetically modified cells using the described protocol, allowing molecular genetic analyses to be performed. Following cell lysis, nucleic acid extraction, and precipitation, the isolated DNA is suitable for digestion by restriction enzymes, amplification by PCR and Southern blotting. This procedure takes approximately 3 h to complete.

A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Publishing Authors By Initials

KE Pilcher,P Fey,P Gaudet,AS Kowal,RL Chisholm,KE Pilcher,P Fey,P Gaudet,AS Kowal,RL Chisholm,

For similar biological sciences: biology: genetics: genomics research abstracts see: biological sciences: biology: genetics: genomics research

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A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 2

Page Numbers: 1325-8

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Keywords Mesh Terms:

KEYWORDS: Genomics

MESH TERMS: methods

Chemical & Substance for Abstract: A reliable general purpose method for extracting genomic DNA from Dictyostelium cells. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for A reliable general purpose method for extracting genomic DNA from Dictyostelium cells.

AFFILIATION: dictyBase, Center for Genetic Medicine, Northwestern University, 676 North Saint Clair Street Suite 1260, Chicago, Illinois 60611, USA.

Country: England

AGENCY: United States NHGRI

GRANT: HG00022

ACRONYM: HG

MEDLINETA: Nat Protoc

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