A rapid method to measure beta-amyloid induced neurotoxicity in vitro.

A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Research Abstract Details 

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A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Abstract Text:

Dhara Patel,Theresa Good,

Beta-amyloid (Abeta) is the primary protein component of senile plaques in Alzheimer's disease (AD) and is believed to be associated with neurotoxicity in the disease. Abeta-induced neurotoxicity is strongly dependent on its structure. The aggregation of the peptide from monomeric form to fibrils is a function of many variables including time. It is because of this dynamic nature of Abeta structure that there is a necessity for an in vitro toxicity assay that is rapid enough to eliminate or at least lower the possibility of Abeta structural changes during the time required for the assay. Here we describe a fast and sensitive method with which to assess Abeta-induced neurotoxicity in SH-SY5Y neuroblastoma cells. The method employs two-color flow cytometry using annexin-phycoerythrin, a marker for cell surface phosphatidylserine that typically indicates early stages of apoptosis, and 7-amino-actinomycin D, a membrane impermeant nucleic acid dye. We compare results using the two-color assay to those obtained using the propidium iodide toxicity assay and demonstrate comparability of results, but in 2h or less with the two-color assay as opposed to 24-48 h with the propidium iodide assay. The assay described could be a useful tool in evaluating the role of Abeta structure in biological activity of the peptide.

A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Publishing Authors By Initials

D Patel,T Good,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neuroscience methods

VOLUME: 161

Page Numbers: 1-10

Journal Abbreviation: J. Neurosci. Methods

ISSN: 0165-0270

DAY: 2

MONTH: 11

YEAR: 2006

A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905558

A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: diagnostic use

Chemical & Substance for Abstract: A rapid method to measure beta-amyloid induced neurotoxicity in vitro. Information

Substance Name: 7-aminoactinomycin D

Registry Number: 7240-37-1

Grant and Affiliation Information for A rapid method to measure beta-amyloid induced neurotoxicity in vitro.

AFFILIATION: Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA. dhara1@umbc.edu

Country: Netherlands

AGENCY: United States NINDS

GRANT: NS042686

ACRONYM: NS

MEDLINETA: J Neurosci Methods

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