A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation.

A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Research Abstract Details 

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A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Abstract Text:

Mun'delanji Vestergaard,Kagan Kerman,Masato Saito,Naoki Nagatani,Yuzuru Takamura,Eiichi Tamiya,

We present the first electrochemical detection, characterization, and kinetic study of the aggregation of Alzheimer's disease (AD) amyloid beta peptides (Abeta-40, Abeta-42) using three different voltammetric techniques at a glassy carbon electrode (GCE). This method is based on detecting changes in the oxidation signal of tyrosine (Tyr) residue. As the peptides aggregate, there are structure conformational changes, which affect the degree of exposure of Tyr to the molecular surface of the peptides. The results show significant differences in the aggregation process between the two peptides, and these correlate highly with established techniques. The method is rapid and label-free, and the principle can be universally applied to other protein aggregation studies related to diseases, such as Huntington's, Parkinson's, and Creutzfeldt Jacob (CJD). This method could also be explored in screening for the effectiveness of AD therapies.

A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Publishing Authors By Initials

M Vestergaard,K Kerman,M Saito,N Nagatani,Y Takamura,E Tamiya,

For similar peptides: peptide fragments research abstracts see: peptides: peptide fragments research

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A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 11892-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 31

MONTH: Aug

YEAR: 2005

A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Keywords Mesh Terms:

KEYWORDS: Peptide Fragments

MESH TERMS: metabolism

Chemical & Substance for Abstract: A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation. Information

Substance Name: Peptide Fragments

Registry Number: 0

Grant and Affiliation Information for A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation.

AFFILIATION: Department of Biological Science and Biotechnology, School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan. munde@jaist.ac.jp

Country: United States

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MEDLINETA: J Am Chem Soc

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