A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations.

A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Research Abstract Details 

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A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Abstract Text:

Bassam M Tashtoush,Elaine L Jacobson,Myron K Jacobson,

A rapid method using an isocratic high-pressure liquid chromatography and UV detection for determination of both all-trans retinoic acid (tretinoin) and 13-cis retinoic acid (isotretinoin) in dermatological preparations is presented. Tretinoin and isotretinoin samples were extracted with acetonitrile by a procedure that can be completed in less than 10 min. Subsequent separation and quantification of amounts as low as 10 pmol was accomplished in less than 15 min using reversed-phase HPLC with isocratic elution with 0.01% trifluoroacetic acid (TFA)/acetonitrile (15:85, v/v). Validation experiments confirmed the precision and accuracy of the method. When applied to commercial tretinoin samples, recoveries of 104.9% for cream formulations and 107.7% for gel formulations were obtained. Application of the method for analysis of a tretinoin cream exposed to solar simulated light (SSL) demonstrated detection of the major photoisomerization product isotretinoin as well as 9-cis retinoic acid, demonstrating the utility of the method for studies of tretinoin photostability. The method should also facilitate studies of the formulation compatibility and photocompatibility of tretinoin with agents that may improve its clinical tolerability.

A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Publishing Authors By Initials

BM Tashtoush,EL Jacobson,MK Jacobson,

For similar organic chemicals: carboxylic acids: acids, acyclic: acetic acids: trifluoroacetic acid research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: acetic acids: trifluoroacetic acid research

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A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 43

Page Numbers: 859-64

Journal Abbreviation:

ISSN: 0731-7085

DAY: 11

MONTH: 10

YEAR: 2006

A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8309336

A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Keywords Mesh Terms:

KEYWORDS: Trifluoroacetic Acid

MESH TERMS: analysis

Chemical & Substance for Abstract: A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations. Information

Substance Name: Trifluoroacetic Acid

Registry Number: 76-05-1

Grant and Affiliation Information for A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations.

AFFILIATION: Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.

Country: England

AGENCY: United States NCI

GRANT: R44 CA-90085

ACRONYM: CA

MEDLINETA: J Pharm Biomed Anal

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