A rapid high-performance liquid chromatography assay of glibenclamide in serum.

A rapid high-performance liquid chromatography assay of glibenclamide in serum. Research Abstract Details 

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A rapid high-performance liquid chromatography assay of glibenclamide in serum. Abstract Text:

A rapid high-performance liquid chromatography (HPLC) determination of glibenclamide in human serum is described. Serum samples to which flufenamic acid has been added as internal standard were treated with acetonitrile as a protein precipitant. After centrifugation, separation and reconstitution, the redissolved residue was eluted from 5 mu Spherisorb C-8 reversed phase column at ambient temperature using a mobile phase consisting of acetonitrile-water (45:55 v/v) at pH 3.7-3.8 and pumped at a flow rate 2 ml/min. The effluent was monitored at 230 nm. The analysis time was no longer than 12 min. A linear relationship between the peak height ratio (glibenclamide/flufenamic acid) and concentration was obtained in the range 20-400 ng/ml. A typical calibration curve has a regression equation y = 0.0035x + 0.015 (r = 0.9999). The detection limit of glibenclamide in serum was 20 ng/ml. The mean recovery of drug from serum samples spiked with known amounts of glibenclamide was 96.77%. Within-day and between-day coefficients of variation were 1.6-4.0% and 1.4-3.5%, respectively. Stability testing indicated that glibenclamide was stable for at least 10 days in serum -20 degrees C. The method developed was applied to determine some pharmacokinetic parameters after the oral administration of 5 mg glibenclamide tablets to a human volunteer.

A rapid high-performance liquid chromatography assay of glibenclamide in serum. Publishing Authors By Initials

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A rapid high-performance liquid chromatography assay of glibenclamide in serum. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of clinical pharmacy and therapeutics

VOLUME: 14

Page Numbers: 181-8

Journal Abbreviation: J Clin Pharm Ther

ISSN: 0269-4727

DAY: 28

MONTH: Jun

YEAR: 1989

A rapid high-performance liquid chromatography assay of glibenclamide in serum. Information

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LANGUAGE: eng

NlmUniqueID: 8704308

A rapid high-performance liquid chromatography assay of glibenclamide in serum. Keywords Mesh Terms:

KEYWORDS: Male

MESH TERMS: pharmacokinetics

Chemical & Substance for Abstract: A rapid high-performance liquid chromatography assay of glibenclamide in serum. Information

Substance Name: Glyburide

Registry Number: 10238-21-8

Grant and Affiliation Information for A rapid high-performance liquid chromatography assay of glibenclamide in serum.

AFFILIATION: Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.

Country: ENGLAND

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MEDLINETA: J Clin Pharm Ther

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