A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis.

A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Research Abstract Details 

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A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Abstract Text:

Qin Fu,Diane E Bovenkamp,Jennifer E Van Eyk,

Serum is a readily available source for diagnostic assays, but the identification of disease-specific serum biomarkers has been impeded by the dominance of human serum albumin (HSA) and immunoglobulin G (IgG) in the serum proteome. Therefore, in order to observe lower-abundance serum proteins, removal or depletion of at least these two proteins is required. However, the depletion method needs to be inexpensive and reproducible. We describe such a protocol that combines delipidation by centrifugation, IgG removal with Protein G Sepharose, and HSA depletion with sodium chloride/ethanol precipitation. The protocol is streamlined to increase reproducibility and is compatible with many proteomic platforms, including two-dimensional gel electrophoresis, and high-performance liquid chromatography either offline or coupled online with a mass spectrometer. The reproducible depletion of lipids, IgG, and HSA permits a higher load of the remaining serum proteins, facilitating the identification of disease biomarkers.

A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Publishing Authors By Initials

Q Fu,DE Bovenkamp,JE Van Eyk,

For similar proteins: albumins: serum albumin research abstracts see: proteins: albumins: serum albumin research

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A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 357

Page Numbers: 365-71

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Keywords Mesh Terms:

KEYWORDS: Serum Albumin

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis. Information

Substance Name: Serum Albumin

Registry Number: 0

Grant and Affiliation Information for A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis.

AFFILIATION: Department of Medicine, Johns Hopkins University, Baltimore, MD, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: N0-HV-28180

ACRONYM: HV

MEDLINETA: Methods Mol Biol

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