[A rapid and high throughput method for apoE genotyping]

[A rapid and high throughput method for apoE genotyping] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[A rapid and high throughput method for apoE genotyping] Abstract Text:

Wen-hong Zhang,Yu-jie Bai,Yan Wang,Ding Li,Xiao-jun Yan,

OBJECTIVE: To develop a new method that can determine the apolipoprotein E(apoE) genotypes rapidly in high throughput. METHODS: Genome DNA samples were extracted from the anticoagulated peripheral blood samples of 79 patients with Alzheimer's disease(AD) and 63 healthy individuals, and the 492 bp apoE gene fragments including 112 and 158 codons were amplified by polymerase chain reaction (PCR). With one PCR product, three recombined alleles (epsilon 2, epsilon 3 and epsilon 4) of apoE gene as controls were obtained by cloning and site-directed mutagenesis. The excess primers and dNTPs in all PCR products were removed by treatment with clean up reagents, then template-directed dye-terminator incorporation reaction (TDI) was performed and R110 or TAMRA labeled Acyclo-terminators were added into the mutation sites specifically. Fluorescence polarization value (FP) was measured using victor 2 multilabel counter and the polymorphisms in 112 and 158 condons of apoE gene were investigated. RESULTS: The apoE genotypes in recombined plasmid controls and all serum samples were analyzed using the authors' TDI-FP method, and the reliability and specificity were confirmed by DNA sequencing. The frequency of epsilon 4 allele in patients was significantly higher than that in controls, suggesting that apoE epsilon 4 allele gene is a risk factor for late-onset AD. CONCLUSION: TDI-FP is an easy, reliable and high throughput technology in analyzing polymorphism of apoE gene; it can be used in the prediction of susceptibility to AD in elderly individuals. Furthermore, it is an ideal method for large-scale screening and for studying the relationship between the allelic and genotypic frequencies of apoE and other diseases.

[A rapid and high throughput method for apoE genotyping] Publishing Authors By Initials

WH Zhang,YJ Bai,Y Wang,D Li,XJ Yan,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[A rapid and high throughput method for apoE genotyping] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yix

VOLUME: 22

Page Numbers: 61-4

Journal Abbreviation: Zhonghua Yi Xue Yi Chuan Xue Z

ISSN: 1003-9406

DAY: 7

MONTH: Feb

YEAR: 2005

[A rapid and high throughput method for apoE genotyping] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9425197

[A rapid and high throughput method for apoE genotyping] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [A rapid and high throughput method for apoE genotyping] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [A rapid and high throughput method for apoE genotyping]

AFFILIATION: Institute of Gene Diagnostic Technique of Chinese PLA, Fourth Military Medical University, Xi'an, Shaanxi, 710032 PR China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhonghua Yi Xue Yi Chuan Xue Z

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A rapid and high throughput method for apoE genotyping Related Publications

• Dietary vitamin E protects the fathead minnow, Pimephales promelas, against noise exposure.
• Processing and cryopreservation for 1963 units of human umbilical cord blood.
• [A basic research to share Fourier transform near-infrared spectrum information resource]
• [A rapid and high throughput method for apoE genotyping]
• [Analysis of resveratrol by pressurized-capillary electrochromatography]
• [C721T mutation of the alpha 1,3 galactosyltransferase gene responsible for Bw subgroup]
• [CALM-AF10 fusion transcripts in primary leukemia with t(10;11) and in vitro chemotherapy sensitivity of leukemic cells with t(10;11)]
• [Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence]
• [Comparative experimental study on treatment of rat's injured sciatic nerve with electroacupuncture and Buyang Huanwu Decoction]
• [Comparison of autologous and allogeneic hematopoietic stem cell transplantation for 140 patients with de novo acute leukemia in first complete remission]
• [Determination of trace rare earth in fossil of dinosaurian egg by laser thermal lens spectrometry]
• [Determinations of indole and methylindole in air by gas chromatography]
• [Effect of soluble anti-CD47 monoclonal antibody on the differentiation and function of human dendritic cells.]
• [Function of eukaryotic transcription factor NF-kappaB in the signal transduction of acute inflammatory response]
• [Influence of FT-NIR spectrometer scanning requirements on the math model's precision]
• [Method of heart rate variability analysis and its application in the field of space medicine]
• [Polymorphisms of six short tandem repeat loci in Zhejiang She ethnic population and Zhejiang Hans of China]
• [Preparation, identification and application of anti-P450scc antibody]
• [Progress on the researches of karyotypes of Chinese spiders]
• [Proliferating cell nuclear antigen expression in recurrent aphthous ulcer and its significance]
• [Rapid manufacturing of degradable porous polymer bone scaffold]
• [Review on job burnout.]
• [Significance of microvascular density and vascular endothelial growth factor in breast cancer]
• [Studies on PANK2 gene mutations in Chinese patients with Hallervorden-Spatz syndrome]
• [Studies on expansion ex vivo of murine bone marrow cells and its hematopoietic reconstitution capacity]
• [Study on the hypotensive effect of manascus]
• [Study on viscosity property of gastrointestinal mucus]
• [The crude drugs of special location of Sichuan as viewed from the tribute drugs in the Tang dynasty]
• [The survey of HIV prevalence among children between 0 to 7 years old in one county in China]
• [The synthesis and absorption spectrum of [Fe(C60)] x ClO]