A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle.

A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Research Abstract Details 

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A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Abstract Text:

J M Coenen Schimke,O H Ljungqvist,G Sarkar,C A Conover,K S Nair,

Insulin-like growth factor-I and -II (IGF-I and IGF-II) and their binding proteins are important components in growth promotion and tissue maintenance. We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. In the first protocol, (control) subjects were given water. In the second protocol, half of these subjects drank Polycose (carbohydrate) and the remaining subjects drank equal calories as a mixed meal. Quadriceps muscle biopsies were taken at 10 h. A semi-quantitative polymerase chain reaction was designed to measure gene expression. IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle, but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin.

A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Publishing Authors By Initials

JM Coenen Schimke,OH Ljungqvist,G Sarkar,CA Conover,KS Nair,

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A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Growth hormone & IGF research : official journal o

VOLUME: 9

Page Numbers: 179-86

Journal Abbreviation: Growth Horm. IGF Res.

ISSN: 1096-6374

DAY: 19

MONTH: Jun

YEAR: 1999

A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Information

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LANGUAGE: eng

NlmUniqueID: 9814320

A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Keywords Mesh Terms:

KEYWORDS: Reverse Transcriptase Polymerase Chain R

MESH TERMS: methods

Chemical & Substance for Abstract: A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle. Information

Substance Name: Insulin-Like Growth Factor II

Registry Number: 67763-97-7

Grant and Affiliation Information for A quantitative PCR measurement of messenger RNA expression of IGF-I, IGF-II and IGFBP-5 in human skeletal muscle.

AFFILIATION: Endocrine Research Unit, Division of Endocrinology and Metabolism, Rochester, MN 55905, USA.

Country: SCOTLAND

AGENCY: United States NCRR

GRANT: RR109

ACRONYM: RR

MEDLINETA: Growth Horm IGF Res

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