A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells.

A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Research Abstract Details 

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A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Abstract Text:

Dachun Wang,David L Haviland,Alan R Burns,Eva Zsigmond,Rick A Wetsel,

Alveolar epithelial type II (ATII) cells are small, cuboidal cells that constitute approximately 60% of the pulmonary alveolar epithelium. These cells are crucial for repair of the injured alveolus by differentiating into alveolar epithelial type I cells. ATII cells derived from human ES (hES) cells are a promising source of cells that could be used therapeutically to treat distal lung diseases. We have developed a reliable transfection and culture procedure, which facilitates, via genetic selection, the differentiation of hES cells into an essentially pure (>99%) population of ATII cells (hES-ATII). Purity, as well as biological features and morphological characteristics of normal ATII cells, was demonstrated for the hES-ATII cells, including lamellar body formation, expression of surfactant proteins A, B, and C, alpha-1-antitrypsin, and the cystic fibrosis transmembrane conductance receptor, as well as the synthesis and secretion of complement proteins C3 and C5. Collectively, these data document the successful generation of a pure population of ATII cells derived from hES cells, providing a practical source of ATII cells to explore in disease models their potential in the regeneration and repair of the injured alveolus and in the therapeutic treatment of genetic diseases affecting the lung.

A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Publishing Authors By Initials

D Wang,DL Haviland,AR Burns,E Zsigmond,RA Wetsel,

For similar peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research abstracts see: peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research

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A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 4449-54

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 2

MONTH: 03

YEAR: 2007

A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin

MESH TERMS: metabolism

Chemical & Substance for Abstract: A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Information

Substance Name: Cystic Fibrosis Transmembrane Conductanc

Registry Number: 126880-72-6

Grant and Affiliation Information for A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells.

AFFILIATION: Research Center for Immunology and Autoimmune Diseases, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas Health Science Center, 1825 Pressler Street, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL 074333

ACRONYM: HL

MEDLINETA: Proc Natl Acad Sci U S A

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