A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood.

A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Research Abstract Details 

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A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Abstract Text:

Dan G Duda,Kenneth S Cohen,David T Scadden,Rakesh K Jain,Dan G Duda,Kenneth S Cohen,David T Scadden,Rakesh K Jain,

Blood circulating endothelial cells (CECs) and circulating hematopoietic progenitor cells (CPCs) represent two cell populations that are thought to play important roles in tissue vascularization. CECs and CPCs are currently studied as surrogate markers in patients for more than a dozen pathologies, including heart disease and cancer. However, data interpretation has often been difficult because of multiple definitions, methods and protocols used to evaluate and count these cells by different laboratories. Here, we propose a cytometry protocol for phenotypic identification and enumeration of CECs and CPCs in human blood using four surface markers: CD31, CD34, CD133 and CD45. This method allows further phenotypic analyses to explore the biology of these cells. In addition, it offers a platform for longitudinal studies of these cells in patients with different pathologies. The protocol is relatively simple, inexpensive and can be adapted for multiple flow cytometer types or software. The procedure should take 2-2.5 h, and is expected to detect 0.1-6.0% viable CECs and 0.01-0.20% CPCs within blood mononuclear cell population.

A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Publishing Authors By Initials

DG Duda,KS Cohen,DT Scadden,RK Jain,DG Duda,KS Cohen,DT Scadden,RK Jain,

For similar genetic phenomena: phenotype research abstracts see: genetic phenomena: phenotype research

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A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 2

Page Numbers: 805-10

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Keywords Mesh Terms:

KEYWORDS: Phenotype

MESH TERMS: cytology

Chemical & Substance for Abstract: A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Information

Substance Name: Biological Markers

Registry Number: 0

Grant and Affiliation Information for A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood.

AFFILIATION: Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Country: England

AGENCY: United States NCI

GRANT: R01-CA115767

ACRONYM: CA

MEDLINETA: Nat Protoc

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