A practical guide to microfluidic perfusion culture of adherent mammalian cells.

A practical guide to microfluidic perfusion culture of adherent mammalian cells. Research Abstract Details 

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A practical guide to microfluidic perfusion culture of adherent mammalian cells. Abstract Text:

Lily Kim,Yi-Chin Toh,Joel Voldman,Hanry Yu,

Culturing cells at microscales allows control over microenvironmental cues, such as cell-cell and cell-matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation. Microfluidic perfusion culture in particular allows controlled delivery and removal of soluble biochemical molecules in the extracellular microenvironment, and controlled application of mechanical forces exerted via fluid flow. There are many challenges to designing and operating a robust microfluidic perfusion culture system for routine culture of adherent mammalian cells. The current literature on microfluidic perfusion culture treats microfluidic design, device fabrication, cell culture, and micro-assays independently. Here we systematically present and discuss important design considerations in the context of the entire microfluidic perfusion culture system. These design considerations include the choice of materials, culture configurations, microfluidic network fabrication and micro-assays. We also present technical issues such as sterilization; seeding cells in both 2D and 3D configurations; and operating the system under optimized mass transport and shear stress conditions, free of air-bubbles. The integrative and systematic treatment of the microfluidic system design and fabrication, cell culture, and micro-assays provides novices with an effective starting point to build and operate a robust microfludic perfusion culture system for various applications.

A practical guide to microfluidic perfusion culture of adherent mammalian cells. Publishing Authors By Initials

L Kim,YC Toh,J Voldman,H Yu,

For similar biomechanics: stress, mechanical research abstracts see: biomechanics: stress, mechanical research

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A practical guide to microfluidic perfusion culture of adherent mammalian cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Lab on a chip

VOLUME: 7

Page Numbers: 681-94

Journal Abbreviation:

ISSN: 1473-0197

DAY: 11

MONTH: 05

YEAR: 2007

A practical guide to microfluidic perfusion culture of adherent mammalian cells. Information

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LANGUAGE: eng

NlmUniqueID: 101128948

A practical guide to microfluidic perfusion culture of adherent mammalian cells. Keywords Mesh Terms:

KEYWORDS: Stress, Mechanical

MESH TERMS: methods

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Substance Name: Culture Media

Registry Number: 0

Grant and Affiliation Information for A practical guide to microfluidic perfusion culture of adherent mammalian cells.

AFFILIATION: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Rm 36-824, Cambridge, MA 02139, USA.

Country: England

AGENCY: United States NCRR

GRANT: RR18878

ACRONYM: RR

MEDLINETA: Lab Chip

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