A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands.

A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Research Abstract Details 

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A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Abstract Text:

Tsutomu Tamada,Masayuki Nara,Hiroshi Kanatsuka,Miyuki Nagaoka,Ryoji Koshida,Gen Tamura,Toshio Hattori,

It is known that several second messengers, such as Ca(2+) or cAMP, play important roles in the intracellular pathway of electrolyte secretion in tracheal submucosal gland. However, the participation of cGMP, and therefore nitric oxide (NO), is not well understood. To investigate the physiologic role of NO, we first examined whether tracheal glands can synthesize NO in response to acetylcholine (ACh), and then whether endogenous NO has some effects on the ACh-triggered ionic currents. From the experiments using the NO-specific fluorescent indicator 4,5-diaminofluorescein diacetate salt (DAF-2DA), we found that a physiologically relevant low dose of ACh (100 nM) stimulated the endogenous NO synthesis, and it was almost completely suppressed in the presence of the nonspecific NO synthase (NOS) inhibitor Nomega-Nitro-L-arginine Methyl Ester Hydrochloride (L-NAME) or the neuronal NOS (nNOS)-specific inhibitor 7-Nitroindazole (7-NI). Patch-clamp experiments revealed that both the NOS inhibitors (L-NAME or 7-NI) and cGK inhibitors (KT-5823 or Rp-8-Br-cGMP) partially decreased ionic currents induced by 30 nM of ACh, but not in the case of 300 nM of ACh. Our results indicate that NO can be synthesized through the activation of nNOS endogenously and has potentiating effects on the gland secretion, under a physiologically relevant ACh stimulation. When cells were stimulated by an inadequately potent dose of ACh, which caused an excess elevation in [Ca(2+)](i), the cells were desensitized. Therefore, due to NO, gland cells become more sensitive to calcium signaling and are able to maintain electrolyte secretion without desensitization.

A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Publishing Authors By Initials

T Tamada,M Nara,H Kanatsuka,M Nagaoka,R Koshida,G Tamura,T Hattori,

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

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A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of respiratory cell and molecular

VOLUME: 37

Page Numbers: 357-65

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1044-1549

DAY: 26

MONTH: 04

YEAR: 2007

A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Information

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LANGUAGE: eng

NlmUniqueID: 8917225

A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: secretion

Chemical & Substance for Abstract: A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands. Information

Substance Name: Cyclic GMP-Dependent Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for A potentiating effect of endogenous NO in the physiologic secretion from airway submucosal glands.

AFFILIATION: Department of Infectious and Respiratory Diseases, Tohoku University School of Medicine, 1-1, Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. tamada@rid.med.tohoku.ac.jp

Country: United States

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MEDLINETA: Am J Respir Cell Mol Biol

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