A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.

A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Research Abstract Details 

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A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Abstract Text:

Jan Pielage,Richard D Fetter,Graeme W Davis,

Synaptic connections are established with characteristic, cell type-specific size and spacing. In this study, we document a role for the postsynaptic Spectrin skeleton in this process. We use transgenic double-stranded RNA to selectively eliminate alpha-Spectrin, beta-Spectrin, or Ankyrin. In the absence of postsynaptic alpha- or beta-Spectrin, active zone size is increased and spacing is perturbed. In addition, subsynaptic muscle membranes are significantly altered. However, despite these changes, the subdivision of the synapse into active zone and periactive zone domains remains intact, both pre- and postsynaptically. Functionally, altered active zone dimensions correlate with an increase in quantal size without a change in presynaptic vesicle size. Mechanistically, beta-Spectrin is required for the localization of alpha-Spectrin and Ankyrin to the postsynaptic membrane. Although Ankyrin is not required for the localization of the Spectrin skeleton to the neuromuscular junction, it contributes to Spectrin-mediated synapse development. We propose a model in which a postsynaptic Spectrin-actin lattice acts as an organizing scaffold upon which pre- and postsynaptic development are arranged.

A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Publishing Authors By Initials

J Pielage,RD Fetter,GW Davis,

For similar nervous system: synapses: synaptic membranes research abstracts see: nervous system: synapses: synaptic membranes research

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A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 175

Page Numbers: 491-503

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 6

MONTH: Nov

YEAR: 2006

A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Information

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LANGUAGE: eng

NlmUniqueID: 375356

A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Keywords Mesh Terms:

KEYWORDS: Synaptic Membranes

MESH TERMS: metabolism

Chemical & Substance for Abstract: A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. Information

Substance Name: Spectrin

Registry Number: 12634-43-4

Grant and Affiliation Information for A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.

AFFILIATION: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS047342

ACRONYM: NS

MEDLINETA: J Cell Biol

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