A plate reader-compatible microchannel array for cell biology assays.

A plate reader-compatible microchannel array for cell biology assays. Research Abstract Details 

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A plate reader-compatible microchannel array for cell biology assays. Abstract Text:

Hongmei Yu,Caroline M Alexander,David J Beebe,

The use of microfluidic devices for studying cell biology holds considerable promise when compared to canonical culture systems. However, the lack of compatibility with existing infrastructure hinders the application of microfluidic devices in the life sciences. Here, we present a microchannel cell culture platform having both operational (compatible with plate readers and pipetting) and performance (lower detection limits, controlled microenvironment) advantages. We demonstrated rapid growth assays and immunocytochemistry on mammary epithelial cells (both a cell line and primary cells) in the microchannel arrays. The utilization of ubiquitous pipetting methods and plate reader endpoints lowers the barriers to use. The simplicity and flexibility of the platform, combined with the throughput of automated detection systems, will facilitate the adoption of microfluidic culture systems in biological laboratories.

A plate reader-compatible microchannel array for cell biology assays. Publishing Authors By Initials

H Yu,CM Alexander,DJ Beebe,

For similar investigative techniques: rheology: microfluidics research abstracts see: investigative techniques: rheology: microfluidics research

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A plate reader-compatible microchannel array for cell biology assays. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Lab on a chip

VOLUME: 7

Page Numbers: 388-91

Journal Abbreviation:

ISSN: 1473-0197

DAY: 2

MONTH: 01

YEAR: 2007

A plate reader-compatible microchannel array for cell biology assays. Information

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LANGUAGE: eng

NlmUniqueID: 101128948

A plate reader-compatible microchannel array for cell biology assays. Keywords Mesh Terms:

KEYWORDS: Microfluidics

MESH TERMS: instrumentation

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Grant and Affiliation Information for A plate reader-compatible microchannel array for cell biology assays.

AFFILIATION: Department of Biomedical Engineering, Madison, WI 53706, USA.

Country: England

AGENCY: United States NCI

GRANT: K25 CA104162

ACRONYM: CA

MEDLINETA: Lab Chip

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