A plastic microchip for nucleic acid purification.

A plastic microchip for nucleic acid purification. Research Abstract Details 

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A plastic microchip for nucleic acid purification. Abstract Text:

Yuxin Liu,Nathaniel C Cady,Carl A Batt,

A microchip for purifying nucleic acids from bacterial pathogens was designed and fabricated in plastic. The fabricated plastic microchips were tested for their ability to purify nucleic acids from the bacteria Listeria monocytogenes (L. monocytogenes), Escherichia coli (E. coli), and Salmonella typhimurium (S. typhimurium). These chips were constructed using rapid and low-cost plastic fabrication techniques including hot embossing and plastic casting. Silicon molds fabricated by photolithography and dry etching were used for chip prototyping. Zeonor plastic (poly (cycloolefin) resin) and epoxy microchips were fabricated using hot embossing and plastic casting, respectively. A low temperature sputtering technique was used to coat a layer of silicon dioxide onto the channel region for nucleic acid binding in chaotropic salt solutions. The purification channels contain an array of features to increase the surface area for DNA binding and purification. DNA was quantified with PicoGreen fluorescent dye and the quality of the material as a substrate for polymerase chain reaction (PCR) was tested using target specific primers. DNA could be recovered from the microchip and detected using PCR from a minimum of 10(6) of L. monocytogenes, E. coli, and S. typhimurium cells, respectively. With the simplicity of the plastic chip's fabrication and DNA purification, our microchip makes it ideal for a miniaturized DNA testing system.

A plastic microchip for nucleic acid purification. Publishing Authors By Initials

Y Liu,NC Cady,CA Batt,

For similar inorganic chemicals: minerals: silicon dioxide research abstracts see: inorganic chemicals: minerals: silicon dioxide research

PUBMED ID PMID:

MEDLINE DATE:

A plastic microchip for nucleic acid purification. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biomedical microdevices

VOLUME: 9

Page Numbers: 769-76

Journal Abbreviation:

ISSN: 1387-2176

DAY: 3

MONTH: Oct

YEAR: 2007

A plastic microchip for nucleic acid purification. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100887374

A plastic microchip for nucleic acid purification. Keywords Mesh Terms:

KEYWORDS: Silicon Dioxide

MESH TERMS: chemistry

Chemical & Substance for Abstract: A plastic microchip for nucleic acid purification. Information

Substance Name: Silicon Dioxide

Registry Number: 7631-86-9

Grant and Affiliation Information for A plastic microchip for nucleic acid purification.

AFFILIATION: Department of Physics and Astronomy, Vanderbilt Institute for Integrative Biosystem Research and Education, Vanderbilt University, 6301 Stevenson Center, Nashville, TN 37235, USA. yuxin.liu@vanderbilt.edu

Country: United States

AGENCY: United States PHS

GRANT: 06000002499A

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MEDLINETA: Biomed Microdevices

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