A phylogenomic analysis of the Actinomycetales mce operons.

A phylogenomic analysis of the Actinomycetales mce operons. Research Abstract Details

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A phylogenomic analysis of the Actinomycetales mce operons. Abstract Text:

Nicola Casali,Lee W Riley,

BACKGROUND: The genome of Mycobacterium tuberculosis harbors four copies of a cluster of genes termed mce operons. Despite extensive research that has demonstrated the importance of these operons on infection outcome, their physiological function remains obscure. Expanding databases of complete microbial genome sequences facilitate a comparative genomic approach that can provide valuable insight into the role of uncharacterized proteins. RESULTS: The M. tuberculosis mce loci each include two yrbE and six mce genes, which have homology to ABC transporter permeases and substrate-binding proteins, respectively. Operons with an identical structure were identified in all Mycobacterium species examined, as well as in five other Actinomycetales genera. Some of the Actinomycetales mce operons include an mkl gene, which encodes an ATPase resembling those of ABC uptake transporters. The phylogenetic profile of Mkl orthologs exactly matched that of the Mce and YrbE proteins. Through topology and motif analyses of YrbE homologs, we identified a region within the penultimate cytoplasmic loop that may serve as the site of interaction with the putative cognate Mkl ATPase. Homologs of the exported proteins encoded adjacent to the M. tuberculosis mce operons were detected in a conserved chromosomal location downstream of the majority of Actinomycetales operons. Operons containing linked mkl, yrbE and mce genes, resembling the classic organization of an ABC importer, were found to be common in Gram-negative bacteria and appear to be associated with changes in properties of the cell surface. CONCLUSION: Evidence presented suggests that the mce operons of Actinomycetales species and related operons in Gram-negative bacteria encode a subfamily of ABC uptake transporters with a possible role in remodeling the cell envelope.

A phylogenomic analysis of the Actinomycetales mce operons. Publishing Authors By Initials

N Casali,LW Riley,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: evolution: phylogeny research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: evolution: phylogeny research

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A phylogenomic analysis of the Actinomycetales mce operons. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC genomics

VOLUME: 8

Page Numbers: 60

Journal Abbreviation: BMC Genomics

ISSN: 1471-2164

DAY: 26

MONTH: 02

YEAR: 2007

A phylogenomic analysis of the Actinomycetales mce operons. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100965258

A phylogenomic analysis of the Actinomycetales mce operons. Keywords Mesh Terms:

KEYWORDS: Phylogeny

MESH TERMS: genetics

Chemical & Substance for Abstract: A phylogenomic analysis of the Actinomycetales mce operons. Information

Substance Name: Bacterial Proteins

Registry Number: 0

Grant and Affiliation Information for A phylogenomic analysis of the Actinomycetales mce operons.

AFFILIATION: University of California at Berkeley, School of Public Health, 140 Warren Hall, Berkeley, CA 94720, USA. ncasali@berkeley.edu <ncasali@berkeley.edu>

Country: England