A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release.

A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Research Abstract Details 

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A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Abstract Text:

Ken K Hirata,Xuchu Que,Samuel G Melendez-Lopez,Anjan Debnath,Simona Myers,D Scott Herdman,Esther Orozco,Alok Bhattacharya,James H McKerrow,Sharon L Reed,

Cysteine proteinases are key virulence factors of Entamoeba histolytica that are released during the process of invasion. We used a chemical mutant of E. histolytica strain HM-1:IMSS, clone L6, which is deficient in virulence, phagocytosis, and cysteine proteinase activity to help define the mechanisms of cysteine proteinase release. All cysteine proteinase genes of wild type HM-1 were present in the L6 mutant genome, but three of the major expressed proteinases, ehcp1, ehcp2, and ehcp5 were both transcribed, translated, and released at lower levels in L6. We hypothesized that a central protein such as the calcium binding protein 1, EhCaBP1, which is required for both phagocytosis and exocytosis might be deficient in this mutant. We found that both mRNA and proteinase levels of EhCaBP1 were decreased in L6. These findings provide an important link between phagocytosis, passive release of multiple cysteine proteinases, and attenuated virulence of this E. histolytica mutant.

A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Publishing Authors By Initials

KK Hirata,X Que,SG Melendez-Lopez,A Debnath,S Myers,DS Herdman,E Orozco,A Bhattacharya,JH McKerrow,SL Reed,

For similar biological factors: toxins, biological: virulence factors research abstracts see: biological factors: toxins, biological: virulence factors research

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A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental parasitology

VOLUME: 115

Page Numbers: 192-9

Journal Abbreviation: Exp. Parasitol.

ISSN: 0014-4894

DAY: 20

MONTH: 09

YEAR: 2006

A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Information

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LANGUAGE: eng

NlmUniqueID: 370713

A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Keywords Mesh Terms:

KEYWORDS: Virulence Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release. Information

Substance Name: Ca2+-binding protein-1

Registry Number: EC 5.3.4.-

Grant and Affiliation Information for A phagocytosis mutant of Entamoeba histolytica is less virulent due to deficient proteinase expression and release.

AFFILIATION: Departments of Pathology and Medicine, University of California, San Diego, CA 92103-8416, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01AI49531

ACRONYM: AI

MEDLINETA: Exp Parasitol

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