A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation.

A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Research Abstract Details 

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A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Abstract Text:

Kristin M Bedard,Sarah Daijogo,Bert L Semler,

A significant number of viral and cellular mRNAs utilize cap-independent translation, employing mechanisms distinct from those of canonical translation initiation. Cap-independent translation requires noncanonical, cellular RNA-binding proteins; however, the roles of such proteins in ribosome recruitment and translation initiation are not fully understood. This work demonstrates that a nucleo-cytoplasmic SR protein, SRp20, functions in internal ribosome entry site (IRES)-mediated translation of a viral RNA. We found that SRp20 interacts with the cellular RNA-binding protein, PCBP2, a protein that binds to IRES sequences within the genomic RNAs of certain picornaviruses and is required for viral translation. We utilized in vitro translation in HeLa cell extracts depleted of SRp20 to demonstrate that SRp20 is required for poliovirus translation initiation. Targeting SRp20 in HeLa cells with short interfering RNAs resulted in inhibition of SRp20 protein expression and a corresponding decrease in poliovirus translation. Our data have identified a previously unknown function of an SR protein (i.e., the stimulation of IRES-mediated translation), further documenting the multifunctional nature of this important class of cellular RNA-binding proteins.

A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Publishing Authors By Initials

KM Bedard,S Daijogo,BL Semler,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The EMBO journal

VOLUME: 26

Page Numbers: 459-67

Journal Abbreviation: EMBO J.

ISSN: 0261-4189

DAY: 21

MONTH: 12

YEAR: 2006

A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8208664

A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation.

AFFILIATION: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92694, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI 26765

ACRONYM: AI

MEDLINETA: EMBO J

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