A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C.

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Abstract Text:

Katsuhiko Amimoto,Taichi Noro,Eiji Oishi,Mitsugu Shimizu,

An unknown cytotoxin was identified in the culture supernatant of Clostridium perfringens type C. The cytotoxin, named TpeL, which was purified using mAb-based affinity chromatography, had a lethal activity of 62 minimum lethal dose (MLD) mg(-1) in mice and a cytotoxic activity of 6.2x10(5) cytotoxic units (CU) mg(-1) in Vero cells. The nucleotide sequence of TpeL was determined. The entire ORF had a length of 4953 bases, and the same nucleotide sequence was not recorded in the GenBank/EMBL/DDBJ databases. The molecular mass calculated from the deduced amino acid sequence was 191 kDa, and a signal peptide region was not found within the ORF. The deduced amino acid sequence exhibited 30-39 % homology to Clostridium difficile toxins A (TcdA) and B (TcdB), Clostridium sordellii lethal toxin (TcsL) and Clostridium novyi alpha-toxin (TcnA). The amino acid sequence of TpeL is shorter than these toxins, and the homologous region was located at the N-terminal site. Eighteen strains of C. perfringens types A, B and C were surveyed for the presence of the tpeL gene by PCR. The tpeL gene was detected in all type B (one strain) and C strains (five strains), but not in any type A strains (12 strains). TpeL was detected in culture filtrates of the five type C strains by dot-blot analysis, but not in the type B strain. It was concluded that TpeL is a novel toxin similar to the known large clostridial cytotoxins. Furthermore, the data indicated that TpeL is produced by many C. perfringens type C strains.

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Publishing Authors By Initials

K Amimoto,T Noro,E Oishi,M Shimizu,

For similar cells: cells, cultured: cell line: vero cells research abstracts see: cells: cells, cultured: cell line: vero cells research

PUBMED ID PMID:

MEDLINE DATE:

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 1198-206

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 28

MONTH: Apr

YEAR: 2007

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Keywords Mesh Terms:

KEYWORDS: Vero Cells

MESH TERMS: toxicity

Chemical & Substance for Abstract: A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C. Information

Substance Name: Cytotoxins

Registry Number: 0

Grant and Affiliation Information for A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C.

AFFILIATION: Kyoto Biken Laboratories, Inc., 24-16 Makishima-cho, Uji, Kyoto 611-0041, Japan. por@kyotobiken.co.jp

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C Related Publications

• A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C.
• Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival.
• Analysis of Body Hair Movement in ELF Electric Field Exposure.
• Bronchopleural fistula after lobectomy for lung cancer.
• Characteristics of the estrous cycle of the swamp buffalo under temperate conditions.
• Current usage of three-dimensional computed tomography angiography for the diagnosis and treatment of ruptured cerebral aneurysms.
• Daily expression of mRNAs for the mammalian Clock genes Per2 and clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells.
• Development of a short version of the sense of coherence scale for population survey.
• Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome.
• Effects of parabolic flight on abdominal arterial pressure in conscious rats.
• Endoscopic mucosal resection for gastric epithelial neoplasms: a study of 39 cases with emphasis on the evaluation of specimens and recommendations for optimal pathologic analysis.
• Epidermolysis bullosa acquisita associated with psoriasis vulgaris.
• G1-G2 aggrecan product that can be generated by M-calpain on truncation at Ala709-Ala710 is present abundantly in human articular cartilage.
• Histone deacetylase inhibitors preferentially augment transient transgene expression in human dermal fibroblasts.
• Humanization of autoantigen.
• Integration of enzyme activities into metabolic flux distributions by elementary mode analysis.
• Inter-individual variation of cytochrome P4502J2 expression and catalytic activities in liver microsomes from Japanese and Caucasian populations.
• Minimal invasive direct coronary artery bypass in moyamoya disease.
• Morphological characteristics of the kidney and lung in the neonatal rats observed after 16 days spaceflight.
• Multicentric invasive carcinomas derived from intraductal papillary mucinous neoplasm (IPMN) of the pancreas: report of a case.
• Non-Hodgkin lymphoma preceded by recalcitrant eczema.
• Novel mutation in a fumalate hydratase gene of a Japanese patient with multiple cutaneous and uterine leiomyomatosis.
• Persistent supercurrent atom chip.
• Role of the mitochondrial membrane permeability transition in cell death.
• Stop codon mutations in the flavin-containing monooxygenase 3 (FMO3) gene responsible for trimethylaminuria in a Japanese population.
• Surface rendered three-dimensional MR imaging for the evaluation of trigeminal neuralgia and hemifacial spasm.
• Synthesis of 4-hydroxyisoleucine by the aldolase-transaminase coupling reaction and basic characterization of the aldolase from Arthrobacter simplex AKU 626.
• T-cell receptor signaling to integrins.
• [Development of the aortic baroreflex under conditions of microgravity]
• theta Oscillation in the anterior cingulate and beta-1 oscillation in the medial temporal cortices: a human case report.