A novel technique for NOD2/CARD15 genotyping using PCR-SSP.

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Abstract Text:

Neema P Mayor,Bronwen E Shaw,Satish Keshav,J Alejandro Madrigal,Steven G E Marsh,Neema P Mayor,Bronwen E Shaw,Satish Keshav,J Alejandro Madrigal,Steven G E Marsh,

The Nucleotide-binding Oligomerisation Domain (NOD) 2 protein is encoded by the Caspase Recruitment Domain (CARD) 15 gene and has a critical role in innate immunity. Recent studies have implicated Single Nucleotide Polymorphisms (SNPs) of the NOD2/CARD15 gene with the onset of several Inflammatory Bowel Disorders (Crohn's Disease, Blau syndrome) and the progression of several malignant diseases. The identification of SNPs in the genotypes of donor and recipient pairs prior to haematopoietic stem cell transplantation have also been shown to predict for a worse outcome, specifically causing increases in the incidence and severity of acute Graft-versus-Host disease, disease relapse and mortality. In light of these widespread areas of interest, we have developed a Polymerase Chain Reaction assay using Sequence Specific Primers (PCR-SSP) to identify the three SNPs that have been implicated, (SNPs 8, 12 and 13). The assay has proven to be a rapid and accurate method of performing NOD2/CARD15 genotyping when compared to other techniques described to date.

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Publishing Authors By Initials

NP Mayor,BE Shaw,S Keshav,JA Madrigal,SG Marsh,NP Mayor,BE Shaw,S Keshav,JA Madrigal,SG Marsh,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of immunological methods

VOLUME: 327

Page Numbers: 82-7

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 10

MONTH: 08

YEAR: 2007

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1305440

A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A novel technique for NOD2/CARD15 genotyping using PCR-SSP. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A novel technique for NOD2/CARD15 genotyping using PCR-SSP.

AFFILIATION: Anthony Nolan Research Institute, Royal Free Hospital, UK.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Immunol Methods

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel technique for NOD2/CARD15 genotyping using PCR-SSP Related Publications

• A conserved dileucine motif mediates clathrin and AP-2-dependent endocytosis of the HIV-1 envelope protein.
• A functional approach to transcriptome profiling: linking gene expression patterns to metabolites that matter.
• A novel technique for NOD2/CARD15 genotyping using PCR-SSP.
• Bovine trichomoniasis: Results of a slaughter survey in Colorado and Nebraska.
• Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase.
• Effect of body mass index on apolipoprotein A-I kinetics in middle-aged men and postmenopausal women.
• Evaluation of radiometric faecal culture and direct PCR on pooled faeces for detection of Mycobacterium avium subsp. paratuberculosis in cattle.
• Evidence for coupled motion and hydrogen tunneling of the reaction catalyzed by glutamate mutase.
• Identification of NR1I2 genetic variation using resequencing.
• Impact of pharmacogenomics on clinical practice in oncology.
• Important roles for macrophage colony-stimulating factor, CC chemokine ligand 2, and mononuclear phagocytes in the pathogenesis of pulmonary fibrosis.
• In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.
• Late death after unrelated-BMT for dyskeratosis congenita following conditioning with alemtuzumab, fludarabine and melphalan.
• Letters to the editor.
• Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax ester.
• Mechanism of benzylsuccinate synthase probed by substrate and isotope exchange.
• Microbial biofilm formation in DUWS and their control using disinfectants.
• Nomenclature for factors of the HLA system, update July 2007.
• Nomenclature for factors of the HLA system, update June 2007.
• Nucleolar localization of parafibromin is mediated by three nucleolar localization signals.
• Pharmacogenomics.
• Primary cardiac synovial sarcoma: a case report and brief review of the literature.
• Pyrosequencing applications.
• Reconstructing mammalian membrane architecture by large area cellular tomography.
• Taming the tools of violence.
• The IMGT/HLA and IPD databases.
• The effect of hearing loss on identification of asynchronous double vowels.
• The relation of attachment security to adolescents' paternal and peer relationships, depression, and externalizing behavior.
• The role of the NADPH oxidase complex, p38 MAPK, and Akt in regulating human monocyte/macrophage survival.
• Time-resolved measurements of the photolysis and recombination of adenosylcobalamin bound to glutamate mutase.