A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements.

A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements. Research Abstract Details 

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A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements. Abstract Text:

Tomoko Kawashima,Mizuko Osanai,Ryo Futahashi,Tetsuya Kojima,Haruhiko Fujiwara,

Transposable elements are valuable for somatic and germ-line transformation. However, long interspersed nuclear elements (LINEs) have not been used because of poor information on the transposition mechanism. We have developed a novel gene delivery system combining baculovirus AcNPV and two silkworm LINEs, SART1 and R1, which integrate into specific sequences of telomeric repeats and 28S ribosomal DNA, respectively. When two LINEs containing the enhanced green fluorescent protein gene recombined into AcNPV were infected into fifth instar larvae of the silkworm, we observed target-specific retrotransposition of LINEs at 72h post-infection, using polymerase chain reaction amplification and sequencing. Telomere- and 28S rDNA-specific transposition occurred in all nine tissues tested, including the ovary and testis. This is the first demonstration of site-specific gene delivery in living larvae. Insertion efficiencies were dependent on the virus titer for injection and the host strains of Bombyx mori. Using this system, we successfully detected the intergeneration transmission of retrotransposed sequences. In addition, AcNPV-mediated SART1 also transposed into telomere of another lepidopteran, Orgyia recens, suggesting that this system is useful for a wide variety of AcNPV-infectious insects. Site-specific gene delivery by virus-mediated LINE will be a potential gene therapy tool to avoid harmful unexpected insertions.

A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements. Publishing Authors By Initials

T Kawashima,M Osanai,R Futahashi,T Kojima,H Fujiwara,

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A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Virus research

VOLUME: 127

Page Numbers: 49-60

Journal Abbreviation: Virus Res.

ISSN: 0168-1702

DAY: 10

MONTH: 05

YEAR: 2007

A novel target-specific gene delivery system combining baculovirus and sequence-specific long interspersed nuclear elements. Information

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LANGUAGE: eng

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AFFILIATION: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Bioscience Bldg. 501, Kashiwa, Chiba 277-8562, Japan. kk47507@mail.ecc.u-tokyo.ac.jp

Country: Netherlands

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MEDLINETA: Virus Res

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