A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer.

A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Research Abstract Details 

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A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Abstract Text:

Jian Yu,Tongyu Zhu,Zhirou Wang,Hongyu Zhang,Ziliang Qian,Huili Xu,Baomei Gao,Wei Wang,Lianping Gu,Jun Meng,Jina Wang,Xu Feng,Yixue Li,Xuebiao Yao,Jingde Zhu,Jian Yu,Tongyu Zhu,Zhirou Wang,Hongyu Zhang,Ziliang Qian,Huili Xu,Baomei Gao,Wei Wang,Lianping Gu,Jun Meng,Jina Wang,Xu Feng,Yixue Li,Xuebiao Yao,Jingde Zhu,

PURPOSE: This study aims to provide a better set of DNA methylation markers in urine sediments for sensitive and specific detection of bladder cancer. EXPERIMENTAL DESIGN: Fifty-nine tumor-associated genes were profiled in three bladder cancer cell lines, a small cohort of cancer biopsies and urine sediments by methylation-specific PCR. Twenty-one candidate genes were then profiled in urine sediments from 132 bladder cancer patients (8 cases for stage 0a; 68 cases for stage I; 50 cases for stage II; 4 cases for stages III; and 2 cases for stage IV), 23 age-matched patients with noncancerous urinary lesions, 6 neurologic diseases, and 7 healthy volunteers. RESULTS: Despite six incidences of four genes reported in 3 of 23 noncancerous urinary lesion patients analyzed, cancer-specific hypermethylation in urine sediments were reported for 15 genes (P < 0.05). Methylation assessment of an 11-gene set (SALL3, CFTR, ABCC6, HPR1, RASSF1A, MT1A, RUNX3, ITGA4, BCL2, ALX4, MYOD1, DRM, CDH13, BMP3B, CCNA1, RPRM, MINT1, and BRCA1) confirmed the existing diagnosis of 121 among 132 bladder cancer cases (sensitivity, 91.7%) with 87% accuracy. Significantly, more than 75% of stage 0a and 88% of stage I disease were detected, indicating its value in the early diagnosis of bladder cancer. Interestingly, the cluster of reported methylation markers used in the U.S. bladder cancers is distinctly different from that identified in this study, suggesting a possible epigenetic disparity between the American and Chinese cases. CONCLUSIONS: Methylation profiling of an 11-gene set in urine sediments provides a sensitive and specific detection of bladder cancer.

A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Publishing Authors By Initials

J Yu,T Zhu,Z Wang,H Zhang,Z Qian,H Xu,B Gao,W Wang,L Gu,J Meng,J Wang,X Feng,Y Li,X Yao,J Zhu,J Yu,T Zhu,Z Wang,H Zhang,Z Qian,H Xu,B Gao,W Wang,L Gu,J Meng,J Wang,X Feng,Y Li,X Yao,J Zhu,

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A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical cancer research : an official journal of

VOLUME: 13

Page Numbers: 7296-304

Journal Abbreviation: Clin. Cancer Res.

ISSN: 1078-0432

DAY: 15

MONTH: Dec

YEAR: 2007

A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Information

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LANGUAGE: eng

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AFFILIATION: Authors' Affiliations: Cancer Genetics and Gene Therapy Program, The State Key Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute and Epigenomics Program, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University.

Country: United States

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MEDLINETA: Clin Cancer Res

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