A novel screening system for self-mRNA targeting proteins.

A novel screening system for self-mRNA targeting proteins. Research Abstract Details 

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A novel screening system for self-mRNA targeting proteins. Abstract Text:

Bei-Wen Ying,Tsutomu Suzuki,Yoshihiro Shimizu,Takuya Ueda,

Here we describe the application of an in vitro translation system for genetic screening, to identify RNA-binding proteins that bind to their own mRNAs. It is a relatively novel system designed using an advanced cell-free translation system reconstructed with purified translational components. Due to the absence of nucleases and proteases, the complex of mRNA and nascent polypeptide synthesized in this system is expected to exhibit high stability ensuring the following efficient selection toward the protein. Escherichia coli ribosomal protein S15, which is known to bind to its own mRNA, was employed as a model molecule to evaluate the system. Wild-type S15 mRNA specifically isolated from a mutant mRNA lacking the secondary structure responsible for binding the S15 protein accumulated markedly after several rounds of selection-amplification. The success of this selection demonstrates the potentiality of the systematic screening of self-mRNA targeting proteins through direct and functional selection. This strategy as a method to identify peptides or proteins that bind to their own mRNAs, is of general interest and has different potential applications, such as, the identification of new regulatory proteins or peptide motifs for RNA recognition, the study of self-mRNA-protein interactions, etc.

A novel screening system for self-mRNA targeting proteins. Publishing Authors By Initials

BW Ying,T Suzuki,Y Shimizu,T Ueda,

For similar proteins: ribosomal proteins research abstracts see: proteins: ribosomal proteins research

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A novel screening system for self-mRNA targeting proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 133

Page Numbers: 485-91

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 2003

A novel screening system for self-mRNA targeting proteins. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A novel screening system for self-mRNA targeting proteins. Keywords Mesh Terms:

KEYWORDS: Ribosomal Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel screening system for self-mRNA targeting proteins. Information

Substance Name: ribosomal protein S15

Registry Number: 0

Grant and Affiliation Information for A novel screening system for self-mRNA targeting proteins.

AFFILIATION: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562.

Country: Japan

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MEDLINETA: J Biochem

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