A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus.

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Abstract Text:

T Matsushita,Y Amagai,K Terai,T Kojima,M Obinata,S Hashimoto,

The suprachiasmatic nucleus of the anterior hypothalamus is the center of an internal biological clock in mammals. Glutamate is the neurotransmitter of retino-hypothalamic tract responsible for mediating the circadian actions of light in rodents. N-methyl-d-aspartate receptors, particularly NR2B subunit are reported to be principally involved in photic resetting of the biological clock in vivo and in slice culture. But, the precise cellular mechanisms of the resetting are not elucidated, because no adequate neuronal cell lines derived from the suprachiasmatic nucleus have been established. We established a neuronal cell line, N14.5, derived from the suprachiasmatic nucleus of a transgenic rat harboring the temperature-sensitive simian virus 40 large T-antigen gene. When the cells were cultured at 39 degrees C, the morphological features were turned fibroblastic into neuronal round cell body with neurite extensions. These cells showed immunoreactivities for neuronal markers (betaIII-tubulin, microtubule-associated protein 2 and TAU2) and as well as for vasoactive intestinal peptide which is expressed in the ventrolateral region of the suprachiasmatic nucleus. The cells expressed N-methyl-d-aspartate receptors, particularly NR1 and NR2B subunits as revealed by quantitative PCR. N-methyl-d-aspartate activated phosphorylation of p44/42 mitogen-activated protein kinase and increased expression level of Per1 and Per2 mRNA. These results suggest that the N14.5 is a novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus, and that N-methyl-d-aspartate receptors expressed in the cells are a functional receptor. The N14.5 cells may be a useful tool to elucidate numerous chronobiological processes, especially resetting mechanism induced by an external light signal.

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Publishing Authors By Initials

T Matsushita,Y Amagai,K Terai,T Kojima,M Obinata,S Hashimoto,

For similar hormones, hormone substitutes, and hormone antagonists: hormones: gastrointestinal hormones: vasoactive intestinal peptide research abstracts see: hormones, hormone substitutes, and hormone antagonists: hormones: gastrointestinal hormones: vasoactive intestinal peptide research

PUBMED ID PMID:

MEDLINE DATE:

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Neuroscience

VOLUME: 140

Page Numbers: 849-56

Journal Abbreviation: Neuroscience

ISSN: 0306-4522

DAY: 17

MONTH: 04

YEAR: 2006

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7605074

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Keywords Mesh Terms:

KEYWORDS: Vasoactive Intestinal Peptide

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus. Information

Substance Name: Mitogen-Activated Protein Kinase 3

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus.

AFFILIATION: Molecular Medicine Laboratories, Institute for Drug Discovery Research, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba-shi, Ibaraki 305-8585 Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Neuroscience

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus Related Publications

• 3,3-diphenylpentane skeleton as a steroid skeleton substitute: novel inhibitors of human 5alpha-reductase 1.
• A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus.
• A short, formal, biomimetic synthesis of (+/-)-polygalolides A and B.
• Airflow produced by dielectric barrier discharge between asymmetric parallel rod electrodes.
• Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes.
• Breakdown of atheromatous plaque due to shear force from arterial perfusion cannula.
• CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes.
• Cellular distribution of Mr 25,000 protein, a protein partially overlapping phosvitin and lipovitellin 2 in vitellogenin B1, and yolk proteins in Xenopus laevis oocytes and embryos.
• Central type 2 corticotropin-releasing hormone receptor mediates hypothalamic-pituitary-adrenocortical axis activation in the rat.
• Close association of centrosomes to the distal ends of the microbody during its growth, division and partitioning in the green alga Klebsormidium flaccidum.
• Co-activation of the secondary somatosensory and auditory cortices facilitates frequency discrimination of vibrotactile stimuli.
• Deciphering the disease process of schizophrenia: the contribution of cortical gaba neurons.
• Edaravone inhibits collagen-induced arthritis possibly through suppression of nuclear factor-kappa B.
• Effects of Immunomodulatory Derivatives of Thalidomide (IMiDs) and Their Analogs on Cell-Differentiation, Cyclooxygenase Activity and Angiogenesis.
• Estrogenic activity in sediments contaminated by nonylphenol in Tokyo Bay (Japan) evaluated by vitellogenin induction in male mummichogs (Fundulus heteroclitus).
• Experiment of seed germination with low pressure environment simulator.
• Generation of different fates from multipotent muscle stem cells.
• High occupancy of sigma-1 receptors in the human brain after single oral administration of fluvoxamine: a positron emission tomography study using [11C]SA4503.
• Impaired parathyroid hormone response to hypocalcemic stimuli in a patient with hypomagnesemic hypocalcemia.
• Interleukin-6 has differential influence on the ability of adjuvant formulations to potentiate antibody responses to a Plasmodium falciparum blood-stage vaccine.
• Nanoscale metal coordination macrocycles fabricated by using "dimeric" dipyrrins.
• Phospholipase A(2)-mediated superoxide production of murine peritoneal macrophages induced by chrysotile stimulation.
• Plasma levels of DJ-1 as a possible marker for progression of sporadic Parkinson's disease.
• Plasmodium falciparum anti-MSP1-19 antibodies induced by MSP1-42 and MSP1-19 based vaccines differed in specificity and parasite growth inhibition in terms of recognition of conserved versus variant epitopes.
• Pleural dissemination of thymoma showing tumor regression after combined corticosteroid and tacrolimus therapy.
• Recurrent p.N767S mutation in the ATP2A2 gene in a Japanese family with haemorrhagic Darier disease clinically mimicking epidermolysis bullosa simplex with mottled pigmentation.
• The change of plasma endothelin-1 levels before and after surgery with or without Down syndrome.
• Two alloalleles of Xenopus laevis hairy2 gene-evolution of duplicated gene function from a developmental perspective.
• [Development of new drugs targeting glial cells]
• [Stem cell systems in skeletal muscle]