A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells.

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Abstract Text:

Peter T Nelson,Mariangels De Planell-Saguer,Stella Lamprinaki,Marianthi Kiriakidou,Paul Zhang,Una O'Doherty,Zissimos Mourelatos,

Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features.

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Publishing Authors By Initials

PT Nelson,M De Planell-Saguer,S Lamprinaki,M Kiriakidou,P Zhang,U O'Doherty,Z Mourelatos,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

PUBMED ID PMID:

MEDLINE DATE:

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 1787-92

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 24

MONTH: 08

YEAR: 2007

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells.

AFFILIATION: Department of Pathology, University of Kentucky, Lexington, KY 40536, USA. peter.nelson@uky.edu

Country: United States

AGENCY: United States NICHD

GRANT: P30-HD026979

ACRONYM: HD

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells Related Publications

• 2'-O-methyl modification in mouse piRNAs and its methylase.
• A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells.
• An mRNA m7G cap binding-like motif within human Ago2 represses translation.
• Introduction and historical background.
• MicroRNAs: Biogenesis and Molecular Functions.
• Site-specific crosslinking of human microRNPs to RNA targets.
• Small RNAs: The seeds of silence.
• Small regulatory RNAs: biogenesis & functions.
• The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs.