A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray.

A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray. Research Abstract Details 

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A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray. Abstract Text:

Dingdong Zhang,Yan Wang,Yunfei Bai,Qinyu Ge,Yingjuan Qiao,Junfeng Luo,Chao Jia,Zuhong Lu,

BACKGROUND: DNA methylation based techniques are important tools in both clinical diagnostics and therapeutics. But most of these methods only analyze a few CpG sites in a target region. Indeed, difference of site-specific methylation may also lead to a change of methylation density in many cases, and it has been found that the density of methylation is more important than methylation of single CpG site for gene silencing. RESULTS: We have developed a novel approach for quantitative analysis of CpG methylation density on the basis of microarray-based hybridization and incorporation of Cy5-dCTP into the Cy3 labeled target DNA by using Taq DNA Polymerase on microarray. The quantification is achieved by measuring Cy5/Cy3 signal ratio which is proportional to methylation density. This methylation-sensitive technique, termed RMEAM (regional methylation elongation assay on microarray), provides several advantages over existing methods used for methylation analysis. It can determine an exact methylation density of the given region, and has potential of high throughput. We demonstrate a use of this method in determining the methylation density of the promoter region of the tumor-related gene MLH1, TERT and MGMT in colorectal carcinoma patients. CONCLUSION: This technique allows for quantitative analysis of regional methylation density, which is the representative of all allelic methylation patterns in the sample. The results show that this technique has the characteristics of simplicity, rapidness, specificity and high-throughput.

A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray. Publishing Authors By Initials

D Zhang,Y Wang,Y Bai,Q Ge,Y Qiao,J Luo,C Jia,Z Lu,

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A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC genomics

VOLUME: 9

Page Numbers: 59

Journal Abbreviation: BMC Genomics

ISSN: 1471-2164

DAY: 31

MONTH: 01

YEAR: 2008

A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray. Information

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LANGUAGE: eng

NlmUniqueID: 100965258

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Grant and Affiliation Information for A novel method to quantify local CpG methylation density by regional methylation elongation assay on microarray.

AFFILIATION: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China. zdd_7597@seu.edu.cn

Country: England

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MEDLINETA: BMC Genomics

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