A novel method for the determination of synthetic colors in ice cream samples.

A novel method for the determination of synthetic colors in ice cream samples. Research Abstract Details 

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A novel method for the determination of synthetic colors in ice cream samples. Abstract Text:

A simple method has been developed for the extraction, separation, and determination of synthetic colors in ice cream samples. The process involves the breakdown of emulsion by neutral detergents (Triton X-100 and Tween 20) followed by extraction with petroleum ether for removal of fat. The aqueous colored solution obtained is treated with 5% acetic acid, and the uptake of color is carried out by a wool-dyeing technique. The color is eluted from the wool with 5% ammonia solution, the solution is evaporated to dryness, and the residue is dissolved in 60% ethanol for paper chromatography using trisodium citrate-ammonia-water (2 + 5 + 95, w/v/v) as the mobile phase. The colored spots from the paper chromatogram are cut and eluted with 60% ethanol, and the absorbance is measured at the respective lambda maximum corresponding to the Rf value of the appropriate standard. The recoveries of 6 colors, including sunset yellow FCF (SSYFCF), tartrazine, carmoisine, ponceau 4R, brilliant blue FCF (BBFCF), and fast green FCF from spiked samples with either detergent were found to be >90%. However, recoveries of erythrosine were 21 and 65% with Triton X-100 and Tween 20, respectively. Indigo carmine could not be recovered at all because of its fugitive property in 5% ammonia solution, which is used to strip the color from the wool. The sensitivity of the method with the use of Tween 20 is 1 ppm (1 microg/g) for the colors in spiked ice cream samples. With this method, we analyzed samples of 20 branded colored ice cream. The results showed the presence of tartrazine (8.4-43.3 ppm), SSYFCF (23.5-117.6 ppm), carmoisine (traces-53.2 ppm), erythrosine (3.5 ppm), and BBFCF (4.1 ppm) in the ice cream samples. Apart from 2 samples of tuttifruity, all of the ice cream samples showed the presence of permitted synthetic colors below the permissible level of 100 ppm established by the Prevention of Food Adulteration Act of India.

A novel method for the determination of synthetic colors in ice cream samples. Publishing Authors By Initials

For similar chemical actions and uses: specialty uses of chemicals: surface-active agents research abstracts see: chemical actions and uses: specialty uses of chemicals: surface-active agents research

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A novel method for the determination of synthetic colors in ice cream samples. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 87

Page Numbers: 657-63

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

A novel method for the determination of synthetic colors in ice cream samples. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

A novel method for the determination of synthetic colors in ice cream samples. Keywords Mesh Terms:

KEYWORDS: Surface-Active Agents

MESH TERMS: analysis

Chemical & Substance for Abstract: A novel method for the determination of synthetic colors in ice cream samples. Information

Substance Name: Octoxynol

Registry Number: 9002-93-1

Grant and Affiliation Information for A novel method for the determination of synthetic colors in ice cream samples.

AFFILIATION: Food Toxicology Laboratory, Industrial Toxicology Research Centre, PO Box 80, Mahatma Gandhi Marg, Lucknow-226001, India.

Country: United States

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MEDLINETA: J AOAC Int

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