A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique.

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Abstract Text:

S B Sato,Y Sako,S Yamashina,S Ohnishi,

We have developed a novel method for isolating specific endocytic vesicles using magnetic ligands and high-gradient magnetic separation. Ligands were prepared by coating extremely fine ferrite particles (10-20 nm) with bovine serum albumin and then conjugating asialoglycopeptides. These ligands were introduced into rat liver by perfusion at 16 or 37 degrees C, or by injection through the tail vein. The ligand particles were observed as electron-dense small grains in membrane-bound vesicles in Kupffer as well as parenchymal cells by electron microscopy. Livers were taken out, homogenized and lightly centrifuged. The supernatant was pumped into a separator glass tube filled with very fine ferritic stainless steel fibers and placed in a magnetic field of 0.9-2 T. Vesicles containing ferrite particles were collected with a high efficiency (ca. 70% of endocytosed magnetic ligands). About 70% of uptake appeared to be mediated by the asialoglycoprotein receptors. The captured vesicles were practically free from marker enzymes for plasma membranes, endoplasmic reticulum, and Golgi apparatus. Lysosomal enzyme activity of the vesicles increased with the time of perfusion at 37 degrees C but not at 16 degrees C. Protein composition of the captured vesicles was analyzed by one- and two-dimensional gel electrophoresis. The composition changed characteristically with time on perfusion at 16 and 37 degrees C. The present method provides a powerful tool to collect prelysosomal endocytic vesicles containing specific ligands and lysosomes fused with these specific endocytic vesicles.

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Publishing Authors By Initials

SB Sato,Y Sako,S Yamashina,S Ohnishi,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

PUBMED ID PMID:

MEDLINE DATE:

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 1481-92

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1986

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique. Information

Substance Name: ferrite

Registry Number: 1317-54-0

Grant and Affiliation Information for A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique Related Publications

• A novel method for isolating specific endocytic vesicles using very fine ferrite particles coated with biological ligands and the high-gradient magnetic separation technique.
• Calcium-induced calcium release from fragmented sarcoplasmic reticulum.
• Enzymatic synthesis of the glucoside of protocatechuic acid in the cockroach, Periplaneta americana.
• Indirect influences of radiation on unirradiated cells through irradiated cells.
• Ki: a key to transform the century of death to the century of life.
• Kinetic studies on the chemical modification of lysozyme by N-bromosuccinimide and its protection by substrates and analogs.
• Lateral mobility of erythrocyte membrane proteins studied by the fluorescence photobleaching recovery technique.
• Loss of osteopontin perturbs the epithelial-mesenchymal transition in an injured mouse lens epithelium.
• Mathematical identification of critical reactions in the interlocked feedback model.
• Modifying effects of fermented brown rice on fecal microbiota in rats.
• Nitric oxide radicals choreograph a radioadaptive response.
• PML-RARalpha inhibitors (ATRA, tamibaroten, arsenic troxide) for acute promyelocytic leukemia.
• Phosphatidylcholine transfer activity in human erythrocyte hemolysate.
• Predictive value of bone marrow accumulation of Tc-99m tetrofosmin for subsequent development of distant metastases in breast cancer.
• Prevention of melanin formation by yeast cerebroside in B16 mouse melanoma cells.
• Protein-lipid interaction in rhodopsin recombinant membranes as studied by protein rotational mobility and lipid alkyl chain flexibility measurements.
• Studies on cytochrome b. 3. Comparison of cytochrome b's from beef heart muscle and larvae of the housefly.
• Studies on cytochrome b. I. Isolation, purification and some properties of cytochrome b from beef heart muscle.
• Studies on cytochrome b. II. Crystallization and some properties of cytochrome b from larvae of the housefly, Musca domestica L.
• Studies on the hydrolytic enzyme models.
• The Nishino Breathing Method and Ki-energy (Life-energy): A Challenge to Traditional Scientific Thinking.
• The effect of clinorotation on vestibular compensation in upside-down swimming catfish.
• The role of the p53 molecule in cancer therapies with radiation and/or hyperthermia.
• Transient acantholytic dermatosis associated with B symptoms of follicular lymphoma.
• [An Advance in the Treatment of CML.]
• [Drug-induced pericarditis]
• [MIR experiment for radiation biology effect: rpsL mutation induction in B. subtilis spore]
• [Postural control of upside-down catfish under the microgravity produced by parabolic flight]
• [The effects of microgravity on induced mutation in E. coli and yeast]
• [Topical steroid injection for refractory oral chronic graft-versus-host disease]