A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry.

A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Research Abstract Details 

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A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Abstract Text:

The fluorescence intensity of the trihydroxyl-phenylfluorone-molybdenum(VI) [Mo(VI)] complex is quenched by protein. Based on this, a novel method for protein assay in aqueous solution was developed. With pH 3.75 acetic acid-sodium acetate buffer solution, in the presence of p-octyl polyethylene glycol phenyl ether microemulsion, the quenched fluorescence intensity is proportional to the concentration of bovine serum albumin (BSA) in the range of 0-7.00 microg/mL, and the detection limit of BSA is 5.65 ng/mL. There is no interference from amino acids and most metal ions. The method developed in this paper has been used for the successful determination of protein in human serum.

A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Publishing Authors By Initials

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 90

Page Numbers: 238-43

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry. Information

Substance Name: Molybdenum

Registry Number: 7439-98-7

Grant and Affiliation Information for A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry.

AFFILIATION: Jinan University, School of Chemistry and Chemical Engineering, Jinan 250022, People's Republic of China (PRC).

Country: United States

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MEDLINETA: J AOAC Int

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