A novel flow cytometric assay for evaluating cell-mediated cytotoxicity.

A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Research Abstract Details 

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A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Abstract Text:

Comprehensive evaluation of cell-mediated cytotoxicity is very important, especially in the clinical setting, when a surrogate immunologic endpoint that correlates with a clinical outcome needs to be defined. With the objective of simultaneously evaluating target cell death and effector cell frequency, the authors combined the measuring of the expression of the degranulation marker CD107a by effector cells with the apoptosis marker annexin V binding to target cells. Using human cytotoxic T lymphocytes, the authors found a significant incubation time-dependent increase of surface CD107a expression on effector cells due to degranulation. A parallel increase of annexin V binding to target cells due to target cell apoptosis was also found. These two parameters have shown excellent correlation in all effector/target cell systems studied. To find possible connections between effector cell degranulation (CD107a expression), granzyme B secretion, and target cell death (annexin V binding), the GrB ELISPOT assay and flow cytometric assay were performed and excellent cross-correlation was found between all three parameters. The specificity of the assay was also shown. These data show that this novel assay allows measurement of cytolytic cell activation and frequency as well as target cell death in the same sample.

A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Publishing Authors By Initials

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PUBMED ID PMID:

MEDLINE DATE: 2005 Jul-Aug

A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of immunotherapy (Hagerstown, Md. : 1997)

VOLUME: 28

Page Numbers: 396-402

Journal Abbreviation: J. Immunother.

ISSN: 1524-9557

DAY: 18

MONTH: 03

YEAR: 2008

A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Information

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LANGUAGE: eng

NlmUniqueID: 9706083

A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. Information

Substance Name: Serine Endopeptidases

Registry Number: EC 3.4.21.-

Grant and Affiliation Information for A novel flow cytometric assay for evaluating cell-mediated cytotoxicity.

AFFILIATION: Laboratory of Cell-Mediated Immunity, Clinical Services Program, SAIC-Frederick, Inc., Frederick, Maryland 21702, USA.

Country: United States

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MEDLINETA: J Immunother

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