A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization.

A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Research Abstract Details 

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A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Abstract Text:

Hua-Ping Li,Zheng Hu,Jiang-Lan Yuan,Han-Dong Fan,Wei Chen,Shi-Jia Wang,Shan-Shan Zheng,Zhong-Liang Zheng,Guo-Lin Zou,Hua-ping Li,Zheng Hu,Jiang-lan Yuan,Han-dong Fan,Wei Chen,Shi-jia Wang,Shan-shan Zheng,Zhong-liang Zheng,Guo-lin Zou,Hua-ping Li,Zheng Hu,Jiang-lan Yuan,Han-dong Fan,Wei Chen,Shi-jia Wang,Shan-shan Zheng,Zhong-liang Zheng,Guo-lin Zou,

A novel serine protease with fibrinolytic activity named CSP was purified from the culture supernatant of the fungus Cordyceps sinensis, a kind of Chinese herbal medicine. Analysis of the purified enzyme by SDS-PAGE indicated that CSP was a single polypeptide chain with an apparent molecular weight of 31 kDa, and N-terminal sequencing revealed that the first ten amino acid residues of the enzyme were Ala-Leu-Ala-Thr-Gln-His-Gly-Ala-Pro-Trp-. When casein was used as a substrate, the proteolytic activity of CSP reached its maximum at pH 7.0 and 40 degrees C. The effect of chemical agents on the enzyme activity indicated that CSP is a serine protease with a free cysteine residue near the active site. It hydrolysed fibrinogen, fibrin and casein with a high efficiency, while hydrolysing bovine serum albumin (BSA) and human serum albumin (HSA) to a lesser extent. CSP was found to be a plasmin-like protease, but not a plasminogen activator, and it preferentially cleaved the A alpha chain of fibrinogen and the alpha-chain of fibrin. Therefore, the extracellular protein CSP may represent a potential new therapeutic agent for the treatment of thrombosis.

A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Publishing Authors By Initials

HP Li,Z Hu,JL Yuan,HD Fan,W Chen,SJ Wang,SS Zheng,ZL Zheng,GL Zou,HP Li,Z Hu,JL Yuan,HD Fan,W Chen,SJ Wang,SS Zheng,ZL Zheng,GL Zou,HP Li,Z Hu,JL Yuan,HD Fan,W Chen,SJ Wang,SS Zheng,ZL Zheng,GL Zou,

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A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Phytotherapy research : PTR

VOLUME: 21

Page Numbers: 1234-41

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ISSN: 0951-418X

DAY: 6

MONTH: Dec

YEAR: 2007

A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization. Information

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LANGUAGE: eng

NlmUniqueID: 8904486

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Grant and Affiliation Information for A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis: purification and characterization.

AFFILIATION: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, 430072, PR China.

Country: England

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MEDLINETA: Phytother Res

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