A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori.

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Abstract Text:

Shoichi Higo,Kazutomo Ori,Hirofumi Takeuchi,Hiromitsu Yamamoto,Tomoaki Hino,Yoshiaki Kawashima,

PURPOSE: The gastric mucoadhesive property of tetracycline-sucralfate acidic complex (CO) was evaluated by using a novel method in vitro to compare with the in vivo test. The mucoadhesive mechanism of the acidic complex was also studied. METHODS: The gastric mucosa removed from a rat was placed covering the end of a plunger and secured in a disposable syringe. The acidic test medium was gradually infused in and then flowed out. Two different kinds of CO, tetracycline, or a physical mixture (PM) were introduced into the device to compare their mucoadhesive properties. The tetracycline content in the residue on the mucosa was measured. The results were compared with those of the in vivo test. The acidic response of CO and the protein binding capacity of a sucrose octasulfate group (SOS) in sucralfate or CO were evaluated. RESULTS: The mucoadhesive properties of CO were clearly superior to those of PM. The remaining amounts of tetracycline in each test sample, determined by the in vitro test, were in agreement with those of the in vivo test. The excellent mucoadhesive property of CO appeared to be caused by the rapid response to the acid and resulting mucoadhesive gel formation. Furthermore, the binding capacity of SOS to the protein was clearly greater than that of PM. The excessive acid treatment during the preparation of CO tended to decrease the mucoadhesive property. CONCLUSIONS: CO appeared to be potentially useful for the eradication of Helicobacter pylori because of the direct delivery of tetracycline to the gastric mucosa for an extended period of time.

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Publishing Authors By Initials

S Higo,K Ori,H Takeuchi,H Yamamoto,T Hino,Y Kawashima,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Pharmaceutical research

VOLUME: 21

Page Numbers: 413-9

Journal Abbreviation: Pharm. Res.

ISSN: 0724-8741

DAY: 8

MONTH: Mar

YEAR: 2004

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406521

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori.

AFFILIATION: Product Research Department, Chugai Pharmaceutical Co. Ltd., 1-135 Komakado, Gotemba, Shizuoka, 412-8513, Japan. higosui@chugai-pharm.co.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Pharm Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori Related Publications

• A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2.
• A novel evaluation method of gastric mucoadhesive property in vitro and the mucoadhesive mechanism of tetracycline-sucralfate acidic complex for eradication of Helicobacter pylori.
• A unique specificity of a calcium activated neutral protease indicated in histone hydrolysis.
• Calpain-calpastatin interactions in epidermoid carcinoma KB cells.
• Changes in cellular levels of ATP and its catabolites in ischemic rat liver.
• Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase.
• Comparison of low and high calcium requiring forms of the calcium-activated neutral protease (CANP) from rabbit skeletal muscle.
• Delivery of immunoprophylactics in transgenic plants: current status.
• Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-leucine and tri-leucine.
• Effects of supplemented diacylglycerol rich in docosahexaenoic acid on serum triacylglycerol in a diet-induced hyperlipidemic model of rats are essentially equivalent to those of triacylglycerol rich in docosahexaenoic acid.
• Electronic and optical properties of ladder-type heteraborins.
• Enhanced serum antigen-specific IgG1 and proinflammatory cytokine production in nicotinic acetylcholine receptor alpha7 subunit gene knockout mice.
• Homeobox protein MSX2 acts as a molecular defense mechanism for preventing ossification in ligament fibroblasts.
• Hydrolysis of protamine by calcium-activated neutral protease (CANP).
• Hydrolytic and autolytic behavior of two forms of calcium-activated neutral protease (CANP).
• Imaging of multistep human hepatocarcinogenesis.
• Isolation and characterization of monoclonal antibodies against calcium-activated neutral protease with low calcium sensitivity.
• Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library.
• Modification of directed-loop algorithm for continuous space simulation of bosonic systems.
• PIASxbeta is a key regulator of osterix transcriptional activity and matrix mineralization in osteoblasts.
• Possible involvement of clathrin in neuritogenesis induced by a protease inhibitor (benzyloxycarbonyl-Leu-Leu-Leu-aldehyde) in PC12 cells.
• Purification and characterization of 210,000-dalton inhibitor of calcium-activated neutral protease from rabbit skeletal muscle and its relation to 50,000-dalton inhibitor.
• Purification and characterization of a calcium-activated neutral protease from rabbit skeletal muscle which requires calcium ions of microM order concentration.
• Purification and characterization of an inhibitor of calcium-activated neutral protease from rabbit skeletal muscle: purification of 50,000-dalton inhibitor.
• Roles of sulfated glycans in lymphocyte homing.
• The Drug Release Profile from Calcium-induced Alginate Gel Beads Coated with an Alginate Hydrolysate.
• The neurite-initiating effect of a tripeptide aldehyde protease inhibitor on PC12h cells.
• VIP induces the translocation and degradation of the alpha subunit of Gs protein in rat pituitary GH4C1 cells.
• Visualization of the penetrative and mucoadhesive properties of chitosan and chitosan-coated liposomes through the rat intestine.
• [Development of gene delivery system using PLGA nanospheres]