A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions.

A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. Research Abstract Details 

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A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. Abstract Text:

Shuling Fan,Vanessa Fogg,Qian Wang,Xiao-Wei Chen,Chia-Jen Liu,Ben Margolis,Shuling Fan,Vanessa Fogg,Qian Wang,Xiao-Wei Chen,Chia-Jen Liu,Ben Margolis,

The Crumbs family of apical transmembrane proteins regulates apicobasal polarity via protein interactions with a conserved C-terminal sequence, ERLI. However, one of the mammalian Crumbs proteins, Crumbs3 (CRB3) has an alternate splice form with a novel C-terminal sequence ending in CLPI (CRB3-CLPI). We report that CRB3-CLPI localizes to the cilia membrane and a membrane compartment at the mitotic spindle poles. Knockdown of CRB3-CLPI leads to both a loss of cilia and a multinuclear phenotype associated with centrosomal and spindle abnormalities. Using protein purification, we find that CRB3-CLPI interacts with importin beta-1 in a Ran-regulated fashion. Importin beta-1 colocalizes with CRB3-CLPI during mitosis, and a dominant-negative form of importin beta-1 closely phenocopies CRB3-CLPI knockdown. Knockdown of importin beta-1 blocks targeting of CRB3-CLPI to the spindle poles. Our data suggest an expanded role for Crumbs proteins in polarized membrane targeting and cell division via unique interactions with importin proteins.

A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. Publishing Authors By Initials

S Fan,V Fogg,Q Wang,XW Chen,CJ Liu,B Margolis,S Fan,V Fogg,Q Wang,XW Chen,CJ Liu,B Margolis,

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A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of cell biology

VOLUME: 178

Page Numbers: 387-98

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 23

MONTH: 07

YEAR: 2007

A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. Information

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LANGUAGE: eng

NlmUniqueID: 375356

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Grant and Affiliation Information for A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions.

AFFILIATION: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK069605

ACRONYM: DK

MEDLINETA: J Cell Biol

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