A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface.

A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Research Abstract Details 

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A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Abstract Text:

Y Suda,K Mori,K Bird,D Marques,J Ormsby,S Tanaka,S Koshida,M Nakamura,S Kusumoto,M Sobel,

A new hetero-bifunctional photo crosslinking reagent, 2-(4-azidoanilyl)-4-(4-azabicyclo-[2,2, 2]hexylammonio)-6-morpholino-1,3,5-triazine chloride, was designed to detect and isolate heparin-binding protein(s) that may act as heparin-receptor(s) on the platelet surface. In a preliminary study using ethanol as a model substrate, the reagent was shown to react with the alcoholic hydroxy group under mild conditions and its crosslinking photoreactivity was high. The reagent effectively formed similar covalent bonds with heparin, while preserving its anticoagulant anti-Xa activity. [3H]Heparin labeled with this reagent crosslinked to antithrombin III very specifically but not to ovalbumin, as analyzed by the Bio-imaging Analyzer System (BAS, Fuji Photo Film, Tokyo). Affinity crosslinking of [3H]heparin was then used to detect heparin-binding proteins on the surface of intact platelets. Several discrete protein bands were detected by the BAS-imaging of SDS-PAGE.

A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Publishing Authors By Initials

Y Suda,K Mori,K Bird,D Marques,J Ormsby,S Tanaka,S Koshida,M Nakamura,S Kusumoto,M Sobel,

For similar proteins: albumins: ovalbumin research abstracts see: proteins: albumins: ovalbumin research

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A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 125

Page Numbers: 1011-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1999

A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Keywords Mesh Terms:

KEYWORDS: Ovalbumin

MESH TERMS: chemical synthesis

Chemical & Substance for Abstract: A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface. Information

Substance Name: Ovalbumin

Registry Number: 9006-59-1

Grant and Affiliation Information for A novel crosslinking reagent and its application for the detection and isolation of heparin-binding protein(s) on the platelet surface.

AFFILIATION: Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan. ysuda@chem.sci.osaka-u.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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