A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity.

A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Research Abstract Details 

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A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Abstract Text:

J Niwa,S Ishigaki,M Doyu,T Suzuki,K Tanaka,G Sobue,

We cloned a novel gene, Dorfin (double ring-finger protein), from human spinal cord. The Dorfin mRNA transcript was 4.4 kb and expressed ubiquitously in many organs as well as in the central nervous system, including the spinal cord. Dorfin encoded 838 amino acid protein Dorfin, which contains two RING-finger motifs and an IBR (in between RING-fingers) motif at its N-terminus. Dorfin is a short-lived protein. Treatment with MG132, a potent proteasome inhibitor, resulted in the accumulation of ubiquitinated Dorfin and Dorfin-associated cellular proteins in cultured cells. Dorfin bound specifically with human ubiquitin-conjugating enzymes UbcH7 and UbcH8 through the RING-finger/IBR domain. Partial deletion of the RING-finger/IBR domain eliminated these interaction and ubiquitination activities. These results strongly suggest that Dorfin is a new member of RING-finger type ubiquitin ligase. Dorfin is localized in the centrosome and probably functions in the microtubule organizing centers.

A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Publishing Authors By Initials

J Niwa,S Ishigaki,M Doyu,T Suzuki,K Tanaka,G Sobue,

For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

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A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 281

Page Numbers: 706-13

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 2

MONTH: Mar

YEAR: 2001

A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Information

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LANGUAGE: eng

NlmUniqueID: 372516

A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Keywords Mesh Terms:

KEYWORDS: Ubiquitins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Information

Substance Name: Ubiquitin-Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity.

AFFILIATION: Department of Neurology, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, 466-8550, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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