A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization.

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Abstract Text:

H Atoda,T Morita,

Using affinity chromatography on a column of factor X-Cellulofine, we have isolated a novel blood coagulation factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake). This anticoagulant protein was also purified by chromatography on Sephadex G-75 and S-Sepharose Fast Flow. The yield of the purified protein was approximately 16 mg from 400 mg of crude venom. The purified protein gave a single band on both analytical alkaline disc-gel electrophoresis and SDS-PAGE. This protein had a relative molecular weight (Mr) after SDS-PAGE of 27,000 before reduction of disulfide bonds and 14,000 after reduction of disulfide bonds. The protein prolonged the clotting time induced by kaolin or factor Xa. In the presence of Ca2+, it formed a complex with factor X, the molar ratio being 1 to 1. Similar complex formation was observed with factor Xa and factor IX/factor IXa, but not with other vitamin K-dependent coagulation factors, i.e., prothrombin, factor VII, protein C, protein S, and protein Z. The interaction of this anticoagulant protein with factor IX/factor X was dependent on gamma-carboxyglutamic acid (Gla) domains, since Gla-domainless derivatives of factor X and factor IXa beta' did not interact with this anticoagulant protein.

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Publishing Authors By Initials

H Atoda,T Morita,

For similar natural sciences: chemistry: chemistry, physical: molecular weight research abstracts see: natural sciences: chemistry: chemistry, physical: molecular weight research

PUBMED ID PMID:

MEDLINE DATE:

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 106

Page Numbers: 808-13

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1989

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Keywords Mesh Terms:

KEYWORDS: Molecular Weight

MESH TERMS: metabolism

Chemical & Substance for Abstract: A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization. Information

Substance Name: Factor X

Registry Number: 9001-29-0

Grant and Affiliation Information for A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis (Habu snake): isolation and characterization.

AFFILIATION: Department of Biochemistry, Meiji College of Pharmacy, Tokyo.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A novel blood coagulation factor IX/factor X-binding protein with anticoagulant activity from the venom of Trimeresurus flavoviridis Habu snake: isolation and characterization Related Publications

• Association between experience of stressful life events and muscle-related temporomandibular disorders in patients seeking free treatment in a dental hospital.
• Cerebral hemodynamics during microgravity.
• Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule.
• Cryptadines A and B, novel C(27)N(3)-type pentacyclic alkaloids from Lycopodium cryptomerinum.
• Degradation of arylarsenic compounds by microorganisms.
• Development of a fully automatic scheme for detection of masses in whole breast ultrasound images.
• Efficacy, safety, and cost effectiveness of intravenous midazolam and flunitrazepam for primary insomnia in terminally ill patients with cancer: a retrospective multicenter audit study.
• Features and feedback: enhancing metamnemonic knowledge at retrieval reduces source-monitoring errors.
• Fusion of single-photon emission computed tomography and computed tomography images of sentinel lymph nodes in extramammary Paget's disease of the scrotum.
• Gait analysis in an osteoarthritis patient with restoration of the hip joint space following contralateral total hip arthroplasty: a case report.
• Glucose intolerance during hormonal therapy for prostate cancer.
• High salt culture conditions inhibit serum- and NGF- but not PMA-induced Egr-1 gene transcription in rat C6 glioma cells.
• Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein.
• Molecular dynamics analysis of interfacial structures and sum frequency generation spectra of aqueous hydrogen halide solutions.
• Novel functions of herbal medicines in dendritic cells: role of amomi semen in tumor immunity.
• Plastic alteration of vestibulo-cardiovascular reflex induced by 2 weeks of 3-G load in conscious rats.
• Polygoacetophenoside, A New Acetophenone Glucoside from Polygonum multiflorum1.
• Pordamacrines A and B, alkaloids from Daphniphyllum macropodum.
• Preferential recognition of a microbial metabolite by human Vgamma2Vdelta2 T cells.
• Protein kinase CK2 is a key activator of histone deacetylase in hypoxia-associated tumors.
• Range of motion measurement of an artificial hip joint using CT images.
• Regulation of bone morphogenetic protein-2 expression by endogenous prostaglandin E2 in human mesenchymal stem cells.
• Snake venom components affecting blood coagulation and the vascular system: structural similarities and marked diversity.
• Taxodistines A and B, abietane-type diterpenes from Taxodium distichum.
• Tobacco smoke causes premature skin aging.
• Transforming growth factor-beta stimulates IL-1beta-induced monocyte chemoattractant protein-1 expression in human synovial cells via the ERK/AP-1 pathway.
• [Pericardial cyst]
• [Protective role of HO-1 in oxidative tissue injuries]
• [Recent changes in the incidence of life-threatening events in the operating room: JSA surveys between 2001 and 2005]
• [Resection of cancer of the cardia enabled by combined treatment with S-1 and paclitaxel after esophageal stenting for impaired patency complicating stage IV gastric cancer - a case report]