[A novel binary vector to get marker-free transgenic plant]

[A novel binary vector to get marker-free transgenic plant] Research Abstract Details 

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[A novel binary vector to get marker-free transgenic plant] Abstract Text:

Xia Li,Hai-Bo Weng,Shao-Yin Han,Yu Xi,Ke-Lan Yong,

A novel practical binary vector to get marker-free transgenic plant was constructed. The estrogen-inducible Cre/loxP DNA recombination system was adopted in this system. All non-target genes located between two identical orientation loxP sites could be excised from the transgenic genome by the Cre expression. In order to analyze this system, the target gene, GUS expression box (CaMV35s: :GUS), was inserted in the MCS outside the region franked by two loxP sites. Then it was introduced into the tobaccos. Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation.

[A novel binary vector to get marker-free transgenic plant] Publishing Authors By Initials

X Li,HB Weng,SY Han,Y Xi,KL Yong,

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[A novel binary vector to get marker-free transgenic plant] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Sheng wu gong cheng xue bao = Chinese journal of b

VOLUME: 22

Page Numbers: 550-4

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ISSN: 1000-3061

DAY: 9

MONTH: Jul

YEAR: 2006

[A novel binary vector to get marker-free transgenic plant] Information

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LANGUAGE: chi

NlmUniqueID: 9426463

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AFFILIATION: College of Life Sciences of Shanghai University, Shanghai 200436, China.

Country: China

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MEDLINETA: Sheng Wu Gong Cheng Xue Bao

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