A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis.

A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis. Research Abstract Details 

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A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis. Abstract Text:

Yun Lin,Xiaoping Wu,Yi Shen,Pengcheng Bu,Dongling Yang,Xiyun Yan,Yun Lin,Xiaoping Wu,Yi Shen,Pengcheng Bu,Dongling Yang,Xiyun Yan,

BACKGROUND: An anti-CD146 monoclonal antibody, AA98, has been identified as an inhibitor of tumor angiogenesis. To overcome the inherent immunogenicity of murine antibody as well as to facilitate immunotoxin construction, a single chain AA98 V(H)/L with three-domain fragments was constructed and expressed in mammalian cells. MATERIALS AND METHODS: The genes of the AA98 heavy chain variable region and the light chain were linked with a modified 12 amino acid sequence that was derived from the heavy chain C(H)1 region, thus constituting the three-domain antibody V(H)/L. Soluble AA98 V(H)/L was produced by mammalian cells and purified by affinity chromatography. The specificity of AA98 V(H)/L for the CD146 molecule was detected by ELISA, immunofluorescence staining and flow cytometry. RESULTS: AA98 V(H)/L alone showed anti-angiogenic properties in a chicken chorioallantoic membrane (CAM) assay as the parent mAb AA98 did. CONCLUSION: This newly generated AA98 V(H)/L antibody displays a therapeutic potential for tumor and other angiogenesis disorders, as well as providing a new strategy for antibody engineering for clinical applications.

A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis. Publishing Authors By Initials

Y Lin,X Wu,Y Shen,P Bu,D Yang,X Yan,Y Lin,X Wu,Y Shen,P Bu,D Yang,X Yan,

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PUBMED ID PMID:

MEDLINE DATE: 2007 Nov-Dec

A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Anticancer research

VOLUME: 27

Page Numbers: 4219-24

Journal Abbreviation: Anticancer Res.

ISSN: 0250-7005

DAY: 29

MONTH: 01

YEAR: 2008

A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis. Information

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LANGUAGE: eng

NlmUniqueID: 8102988

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Grant and Affiliation Information for A novel antibody AA98 V(H)/L directed against CD146 efficiently inhibits angiogenesis.

AFFILIATION: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China.

Country: Greece

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MEDLINETA: Anticancer Res

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