A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity.

A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Research Abstract Details 

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A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Abstract Text:

Toshie Okada,Tokihiko Sawada,Tatsushi Osawa,Masakazu Adachi,Keiichi Kubota,Toshie Okada,Tokihiko Sawada,Tatsushi Osawa,Masakazu Adachi,Keiichi Kubota,

BACKGROUND/AIMS: MK615 is an anti-cancer substance extracted from the Japanese apricot. In the present study, the anti-neoplastic effect of MK615 against hepatocellular carcinoma (HCC) was evaluated in vitro, and its mechanism was elucidated. METHODOLOGY: Two HCC lines, HuH7 and Hep3B, were cultured with MK615 at concentrations of 600, 300, 150, and 0 microg/mL. Growth inhibition was evaluated by MTT assay, and killing activity was determined by LDH assay. Cell cycle stages were evaluated by flow cytometry. Expression of Aurora A kinase (Aurora A) was evaluated by real-time PCR and Western blotting, and inhibition of Aurora A activity was determined by HTscan. RESULTS: MK615 inhibited the growth of, and lysed, HuH7 and Hep3B cells in a dose-dependent manner. Cell cycle analysis revealed that MK615 increased the population of cells in G2/M phase. Real-time PCR and Western blotting showed that MK615 suppressed the expression of Aurora A. HTscan assay demonstrated that Aurora A activity was specifically inhibited by 34.3%, 32.9%, and 54.3% at 150, 300, and 600 microg/mL MK615, respectively. CONCLUSIONS: MK615 has an anti-cancer effect against HCC lines in vitro, and the effect is exerted through inhibition of Aurora A activity.

A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Publishing Authors By Initials

T Okada,T Sawada,T Osawa,M Adachi,K Kubota,T Okada,T Sawada,T Osawa,M Adachi,K Kubota,

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A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Hepato-gastroenterology

VOLUME: 54

Page Numbers: 1770-4

Journal Abbreviation: Hepatogastroenterology

ISSN: 0172-6390

DAY: 16

MONTH: Sep

YEAR: 2007

A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Information

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LANGUAGE: eng

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AFFILIATION: Second Department of Surgery, Dokkyo University School of Medicine, Tochigi, Japan.

Country: Greece

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MEDLINETA: Hepatogastroenterology

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