A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays.

A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays. Research Abstract Details 

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A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays. Abstract Text:

Fumin Li,Jacob Maguigad,Mary Pelzer,Xiangyu Jiang,Qin C Ji,

Sensitive and high-throughput bioanalytical assays are of vital importance to drug discovery and development. Ultra-performance liquid chromatography (UPLC), utilizing sub-2-microm particles, greatly increases the separation throughput and efficiency, resulting in LC peaks as narrow as or less than 1 s (full width at half maxima, FWHM). This, however, could pose practical challenges for bioanalytical applications using quadrupole mass spectrometry (MS) to acquire sufficient data points to ensure accurate and reliable quantitation. Here, we present a novel 'peak parking' strategy to reduce the flow rate during UPLC peak elution, therefore extending the useful MS acquisition window. The high-throughput advantage of UPLC is maintained since no significant increase of the overall UPLC run time is needed. This strategy was demonstrated in an assay development for lansoprazole, a gastric proton-pump inhibitor, in human plasma employing liquid-liquid extraction. The method was validated from 50.0 to 50 000 pg/mL. Copyright (c) 2008 John Wiley & Sons, Ltd.

A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays. Publishing Authors By Initials

F Li,J Maguigad,M Pelzer,X Jiang,QC Ji,

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A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Rapid communications in mass spectrometry : RCM

VOLUME: 22

Page Numbers: 486-94

Journal Abbreviation: Rapid Commun. Mass Spectrom.

ISSN: 0951-4198

DAY: 5

MONTH: 02

YEAR: 2008

A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays. Information

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LANGUAGE: eng

NlmUniqueID: 8802365

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Grant and Affiliation Information for A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays.

AFFILIATION: Covance Laboratories, Inc., Bioanalytical Chemistry, 3301 Kinsman Boulevard, Madison, WI 53704, USA.

Country: England

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MEDLINETA: Rapid Commun Mass Spectrom

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