A new quantitative in vitro microculture method for Giardia duodenalis trophozoites.

A new quantitative in vitro microculture method for Giardia duodenalis trophozoites. Research Abstract Details 

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A new quantitative in vitro microculture method for Giardia duodenalis trophozoites. Abstract Text:

Ely , da Luz,Marieke Vermeersch,Paul Cos,Louis Maes,Ely , da Luz,Marieke Vermeersch,Paul Cos,Louis Maes,

A reliable, rapid and low-cost method for drug sensitivity determination of Giardia duodenalis trophozoites (WB-strain) was developed in a 96-well plate. Using a standard inoculum of 5 x 10(4) trophozoites per well (300 microl), good growth was obtained after sealing the plate with an air-tight adhesive tape and incubation at 37 degrees C for 72 h in modified TYI-S-33 medium. Viable burdens were quantified using the formazan dyes MTT (100 microg/well) and XTT (20 microg/well) and the fluorescent substrate resazurin (2.5 microg/well). Prior removal of the culture medium is required since it causes spontaneous reduction of the substrate. Resazurin proved to be far superior to MTT and XTT with a level of sensitivity of about 3 x 10(4) trophozoites. Inhibitory concentrations (IC(50)) of several anti-giardial reference drugs were within the range of published values: metronidazole 2.25 microM, tinidazole 1.75 microM, albendazole 0.10 microM, furazolidone 2.00 microM and quinacrine 0.32 microM. The broad-spectrum antibiotics chloramphenicol, rifampicin, penicillin G+streptomycin and gentamycin were devoid of any inhibitory activity and are considered suitable for decontamination during excystation experiments.

A new quantitative in vitro microculture method for Giardia duodenalis trophozoites. Publishing Authors By Initials

E ,RA da Luz,M Vermeersch,P Cos,L Maes,E ,RA da Luz,M Vermeersch,P Cos,L Maes,

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A new quantitative in vitro microculture method for Giardia duodenalis trophozoites. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiological methods

VOLUME: 71

Page Numbers: 101-6

Journal Abbreviation: J. Microbiol. Methods

ISSN: 0167-7012

DAY: 15

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Laboratory for Microbiology, Parasitology and Hygiene, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.

Country: Netherlands

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MEDLINETA: J Microbiol Methods

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