A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry.

A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Research Abstract Details 

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A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Abstract Text:

Weichuan Yu,Junfeng Liu,Chris Colangelo,Erol Gulcicek,Hongyu Zhao,

Isotope-coded affinity tags (ICAT) is a labeling technique that provides insights into quantitative molecular changes. In this paper, we propose a new protocol to identify and analyze ICAT labeled peak pairs in high-resolution LC-MS data. Our major contributions are: (1) we use isotope distance constraint, ICAT distance constraint, and LC-span constraint to identify ICAT labeled peak pairs and (2) we propose to trigger tandem MS/MS scanning based on the ratio estimation value of identified ICAT peak pairs instead of the peak intensity values. Compared with current approaches that choose peaks with high intensity values for tandem MS/MS scanning, the new protocol can improve the scanning efficiency and accuracy.

A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Publishing Authors By Initials

W Yu,J Liu,C Colangelo,E Gulcicek,H Zhao,

For similar biological sciences: biochemistry: proteomics research abstracts see: biological sciences: biochemistry: proteomics research

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A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Computational biology and chemistry

VOLUME: 31

Page Numbers: 215-21

Journal Abbreviation:

ISSN: 1476-9271

DAY: 20

MONTH: 03

YEAR: 2007

A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Information

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LANGUAGE: eng

NlmUniqueID: 101157394

A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Keywords Mesh Terms:

KEYWORDS: Proteomics

MESH TERMS: methods

Chemical & Substance for Abstract: A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for A new protocol of analyzing isotope-coded affinity tag data from high-resolution LC-MS spectrometry.

AFFILIATION: Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. eeyu@ust.hk

Country: England

AGENCY: United States NIGMS

GRANT: R01 GM 59507

ACRONYM: GM

MEDLINETA: Comput Biol Chem

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