A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin.

A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Research Abstract Details 

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A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Abstract Text:

T Kitagawa,T Kanamaru,H Wakamatsu,H Kato,S Yano,Y Asanuma,

A new method for the preparation of ampicillin-BSA conjugate by a three step procedure was developed. The first step is the introduction of a maleimide residue to ampicillin by a cross-linking reagent, MBS. The second step is reductive cleavage of disulfide bonds in BSA. The third step is thioether formation between the introduced maleimide residues and the reduced thiol groups. The obtained ampicillin-BSA conjugated raised an anti-ampicillin serum in rabbits. A new reagent, MPGS, was used for enzyme labelling of ampicillin to avoid immunological cross reaction. Using the enzyme labelled ampicillin and anti-ampicillin serum, enzyme immunoassay of ampicillin was successful in detecting 4 ng to 1 mug. Cross reactivities of anti-ampicillin to ampicillin analogs were studied by the enzyme immunoassay to find that the antiserum is specific to penicillin especially to ampicillin but hardly reacts with cephalosporins or the penicilloic acid derivative of ampicillin.

A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Publishing Authors By Initials

T Kitagawa,T Kanamaru,H Wakamatsu,H Kato,S Yano,Y Asanuma,

For similar organic chemicals: imides: succinimides research abstracts see: organic chemicals: imides: succinimides research

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A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 84

Page Numbers: 491-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1978

A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Keywords Mesh Terms:

KEYWORDS: Succinimides

MESH TERMS: immunology

Chemical & Substance for Abstract: A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin. Information

Substance Name: Galactosidases

Registry Number: EC 3.2.1.-

Grant and Affiliation Information for A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin.

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Country: JAPAN

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MEDLINETA: J Biochem

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