A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway.

A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. Research Abstract Details 

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A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. Abstract Text:

Simon P Gough,Kamila Rzeznicka,Ragna Peterson Wulff,Jose da Cruz Francisco,Andreas Hansson,Poul Erik Jensen,Mats Hansson,Simon P Gough,Kamila Rzeznicka,Ragna Peterson Wulff,Jose da Cruz Francisco,Andreas Hansson,Poul Erik Jensen,Mats Hansson,

Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide. Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H(2)O/25% NH(3). Isolated MPE was identified by absorption and fluorescence spectroscopy, and its purity was analyzed by HPLC. The extracted MPE was dried and redissolved in buffered DMSO and its substrate activity is shown by enzymatic cyclase assays. A linear time course was observed for MPE conversion to protochlorophyllide by enzymes from barley etioplasts. Our innovation of freeze drying the R. capsulatus cells before extraction provides a high yield method for MPE, which is significantly faster and more reproducible than previous extraction methods.

A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. Publishing Authors By Initials

SP Gough,K Rzeznicka,R Peterson Wulff,Jda Francisco,A Hansson,PE Jensen,M Hansson,SP Gough,K Rzeznicka,R Peterson Wulff,Jda Francisco,A Hansson,PE Jensen,M Hansson,

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A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant physiology and biochemistry : PPB / Société

VOLUME: 45

Page Numbers: 932-6

Journal Abbreviation: Plant Physiol. Biochem.

ISSN: 0981-9428

DAY: 14

MONTH: 09

YEAR: 2007

A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. Information

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LANGUAGE: eng

NlmUniqueID: 9882449

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Grant and Affiliation Information for A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway.

AFFILIATION: Department of Biochemistry, Lund University, Box 124, SE-22100 Lund, Sweden.

Country: France

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MEDLINETA: Plant Physiol Biochem

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