A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes.

A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Research Abstract Details 

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A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Abstract Text:

FTY720 is a unique immunosuppressive drug produced by modification of a metabolite from Isaria sinclairii. In vitro treatment of human mononuclear cells with FTY720 resulted in a dose-dependent reduction of cell viability. These treated cells demonstrated characteristic DNA ladder formation on agarose gel electrophoresis. Jurkat cells transfected with human bcl-2 gene were resistant to FTY720; their neo type was susceptible to the drug. A rapid acceleration of cell death in human mononuclear cells was seen as early as 2 hr after incubation with FTY720. The intracellular Bax protein increased remarkably 1 hr after the culture; it markedly decreased in the surviving cells at 2 and 3 hr. Coincidental to the Bax decrease. Bcl-2 progressively decreased beginning 2 hr after the culture. Thus, the ratio of Bcl-2 to Bax was decreased by the enhanced expression of Bax immediately after FTY720-treatment, resulting in rapid cell death acceleration. The surviving cells (FTY720-resistant cells) at 2 and 3 hr after culture showed a similar ratio of Bcl-2 to Bax as was observed in the control cells. These results suggest that FTY720 displays bcl-2-associated apoptotic cell death in human mononuclear cells.

A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Publishing Authors By Initials

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A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Immunology

VOLUME: 89

Page Numbers: 518-23

Journal Abbreviation: Immunology

ISSN: 0019-2805

DAY: 5

MONTH: Dec

YEAR: 1996

A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374672

A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Keywords Mesh Terms:

KEYWORDS: bcl-2-Associated X Protein

MESH TERMS: analogs & derivatives

Chemical & Substance for Abstract: A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes. Information

Substance Name: fingolimod

Registry Number: 162359-55-9

Grant and Affiliation Information for A new immunosuppressant, FTY720, induces bcl-2-associated apoptotic cell death in human lymphocytes.

AFFILIATION: Department of Experimental Surgery and Bioengineering, National Children's Medical Research Centre, Tokyo, Japan.

Country: ENGLAND

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MEDLINETA: Immunology

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