A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates.

A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Research Abstract Details 

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A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Abstract Text:

Haichen Song,Gloria Ramirez Nieto,Daniel R Perez,

In light of the recurrent outbreaks of low pathogenic avian influenza (LPAI) and highly pathogenic avian influenza (HPAI), there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, we introduced by reverse genetics temperature-sensitive mutations in the PB1 and PB2 genes of an avian influenza virus, A/Guinea Fowl/Hong Kong/WF10/99 (H9N2) (WF10). Further genetic modifications were introduced into the PB1 gene to enhance the attenuated (att) phenotype of the virus in vivo. Using the att WF10 as a backbone, we substituted neuraminidase (NA) for hemagglutinin (HA) for vaccine purposes. In chickens, a vaccination scheme consisting of a single dose of an att H7N2 vaccine virus at 2 weeks of age and subsequent challenge with the wild-type H7N2 LPAI virus resulted in complete protection. We further extended our vaccination strategy against the HPAI H5N1. In this case, we reconstituted an att H5N1 vaccine virus, whose HA and NA genes were derived from an Asian H5N1 virus. A single-dose immunization in ovo with the att H5N1 vaccine virus in 18-day-old chicken embryos resulted in more than 60% protection for 4-week-old chickens and 100% protection for 9- to 12-week-old chickens. Boosting at 2 weeks posthatching provided 100% protection against challenge with the HPAI H5N1 virus for chickens as young as 4 weeks old, with undetectable virus shedding postchallenge. Our results highlight the potential of live att avian influenza vaccines for mass vaccination in poultry.

A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Publishing Authors By Initials

H Song,GR Nieto,DR Perez,

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A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 81

Page Numbers: 9238-48

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 27

MONTH: 06

YEAR: 2007

A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. Information

Substance Name: Neuraminidase

Registry Number: EC 3.2.1.18

Grant and Affiliation Information for A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates.

AFFILIATION: Department of Veterinary Medicine, University of Maryland, College Park, MD 20742-3711, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R21-AI071014

ACRONYM: AI

MEDLINETA: J Virol

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