A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells.

A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Research Abstract Details 

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A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Abstract Text:

T Tsuruo,K Hirayama,H Satoh,T Ukita,

A new form of high molecular weight DNA polymerase [EC 2.7.7.7] (polymerase N) was isolated from the nuclei of rat ascites hepatoma cells. Polymerase C, which was isolated previously from whole cell extract, was also isolated from the nuclei (Tsuruo, T. and Ukita, T. (1974) Biochim. Biophys. Acta 353, 146-159). Polymerase N was not found in the cytoplasmic fraction of the cell, while polymerase C existed in both the nucleus and cytoplasm. The molecular weight of polymerase N (8.7 S) was larger than that of polymerase C (7.4 S). On freezing and thawing, polymerase N was converted to polymerase C. In the nucleus the amount of polymerase N was larger than that of polymerase C. These data suggest that polymerase N, which was specifically present in the nucleus, was a complex form of polymerase C. In in vitro assay, polymerase N showed properties similar to those of polymerase C. Oligoribonucleotide was an effective initiator for the polymerization reaction by polymerase N. The DNA synthesis on single stranded fd phage DNA was greatly stimulated by the concomitant synthesis of RNA.

A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Publishing Authors By Initials

T Tsuruo,K Hirayama,H Satoh,T Ukita,

For similar genetic structures: templates, genetic research abstracts see: genetic structures: templates, genetic research

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A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 78

Page Numbers: 401-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1975

A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Keywords Mesh Terms:

KEYWORDS: Templates, Genetic

MESH TERMS: enzymology

Chemical & Substance for Abstract: A new form of high molecular weight DNA polymerase in the nuclei of rat ascites hepatoma cells. Information

Substance Name: DNA Nucleotidyltransferases

Registry Number: EC 2.7.7.-

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Country: JAPAN

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MEDLINETA: J Biochem

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