A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP.

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Abstract Text:

Y Takebe,M Shibuya,Y Kaziro,

A strain bearing an extragenic suppressor of cya mutation was isolated as a second-site revertant of an adenylate cyclase deficient strain. The mutant was unable to synthesize cAMP but showed normal fermentation profiles and growth properties on a variety of carbon sources. The site of reversion was mapped in, or near, the structural gene for the cAMP receptor protein. Structural alteration of the protein was directly demonstrated by the following biochemical observations: (i) A 10-fold decrease in the dissociation constant for cAMP, (ii) an acidic shift in the isoelectric point, and (iii) the altered binding properties to lambdah80dlac ps DNA.

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Publishing Authors By Initials

Y Takebe,M Shibuya,Y Kaziro,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

PUBMED ID PMID:

MEDLINE DATE:

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 1615-23

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1978

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. Information

Substance Name: Cyclic AMP

Registry Number: 60-92-4

Grant and Affiliation Information for A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A new extragenic suppressor of cya mutation Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP Related Publications

• A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP.
• A new protein factor required for polypeptide elongation in mammalian tissues.
• Binding of aminoacyl-tRNA to ribosomes promoted by elongation factor Tu. Studies on the role of GTP hydrolysis.
• Conformational transition of polypeptide chain elongation factor G as determined by electron spin resonance.
• Conformational transitions of polypeptide chain elongation factor Tu. I. Studies with hydrophobic probes.
• Conformational transitioons of polypeptide chain elongation factor Tu. II. Further studies by electron spin resonance.
• Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli.
• Distribution of the low molecular weight form of eukaryotic elongation factor 1 in various tissues.
• Formation of a binary complex between elongation factor G and guanine nucleotides.
• Further studies on the interaction of the polypeptide chain elongation factor G with guanine nucleotides.
• Further studies on the properties of the polypeptide chain elongation factors Tu and Ts: hydrogen-tritium exchange, optical rotatory dispersion, and intrinsic fluorescence.
• Interactions between elongation factor tu-guanosine triphosphate and ribosomes and the role of ribosome-bound transfer RNA in guanosine triphosphatase reaction.
• Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis.
• Limited hydrolysis of the polypeptide chain elongation factor Tu by trypsin. Isolation and characterization of the polypeptide fragments.
• Mechanism of action of chromomycin A3. 1. Inhibition of nucleic acid metabolism in Bacillus subtilis cells.
• Mechanism of the ribosome-dependent uncoupled GTPase reaction catalyzed by polypeptide chain elongation factor G.
• Proceedings: Polypeptide chain elongation in bacterial and mammalian systems.
• Properties and function of the sulfhydryl group in the polypeptide chain elongation factor G from E. coli.
• Purification and properties of polypeptide chain elongation factor-1 beta gamma from pig liver.
• Purification and properties of polypeptide chain elongation factor-1alpha from pig liver.
• Purification and properties of the polypeptide chain release factor (RF-4) from an extreme thermophile, Thermus thermophilus HB8.
• Purification of tubulin from bovine brain and its interaction with guanine nucleotides.
• Role of GTP in the assembly of microtubules.
• Studies on amino-acyl-sRNA synthetases from Pseudomonas and methionyl-sRNA. I. Partial purification of leucyl-, phenylalanyl-, tyrosyl-, and synthetases.
• Studies on polypeptide elongation factor 2 from pig liver. I. Purification and properties.
• Studies on polypeptide elongation factor 2 from pig liver. III. Interaction with guanine nucleotides in the presence and absence of ribosomes.
• Studies on the polypeptide elongation factors form E. coli. VI. Characterization of sulfhydryl groups in EF-Tu and EF-Ts.
• Studies on the polypeptide elongation factors from E. coli. IV. Crystalline Tu-GTP, Tu-Gpp(CH2)p, and phenylalanyl-tRNA-Tu-GTP complex.
• Studies on the polypeptide elongation factors from E. coli. V. Properties of various complexes containing EF-Tu and EF-Ts.
• Translocation reaction promoted by polypeptide chain elongation factor-2 from pig liver.