A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate.

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Research Abstract Details 

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A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Abstract Text:

Steven B Larson,John S Day,Robert Cudney,Alexander McPherson,

The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at room temperature in a previously unreported unit cell belonging to space group P3(1). There are two molecules of nucleotide per enzyme molecule, one of which lies in the active-site cleft in the productive binding mode, whilst the guanine base of the other dGMP occupies the pyrimidine-specific binding site in a nonproductive mode such that it forms hydrogen bonds to the phosphate group of the first dGMP. This is the first RNase A structure containing a guanine base in the B2 binding site. Each dGMP molecule is involved in intermolecular interactions with adjacent RNase A molecules in the lattice and the two nucleotides appear to direct the formation of the crystal lattice. Because GMP may be produced during degradation of RNA, this association could represent an inhibitor complex and thereby affect the observed enzyme kinetics.

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Publishing Authors By Initials

SB Larson,JS Day,R Cudney,A McPherson,

For similar investigative techniques: chemistry, analytical: crystallography: x-ray diffraction research abstracts see: investigative techniques: chemistry, analytical: crystallography: x-ray diffraction research

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MEDLINE DATE:

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 63

Page Numbers: 728-33

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 31

MONTH: 08

YEAR: 2007

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101226117

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Keywords Mesh Terms:

KEYWORDS: X-Ray Diffraction

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate. Information

Substance Name: Ribonuclease, Pancreatic

Registry Number: EC 3.1.27.5

Grant and Affiliation Information for A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate.

AFFILIATION: Department of Molecular Biology and Biochemistry, The University of California, Irvine, CA 92697-3900, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM074899

ACRONYM: GM

MEDLINETA: Acta Crystallogr Sect F Struct

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ACCESSION NUMBER: R2QCASF

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