A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display.

A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Research Abstract Details 

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A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Abstract Text:

Xiang-Bin Wang,Bing Zhou,Chang-Cheng Yin,Qing Lin,Hua-Liang Huang,

Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti-4-1BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping.

A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Publishing Authors By Initials

XB Wang,B Zhou,CC Yin,Q Lin,HL Huang,

For similar investigative techniques: genetic techniques: sequence alignment research abstracts see: investigative techniques: genetic techniques: sequence alignment research

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A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 136

Page Numbers: 19-28

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 2004

A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Keywords Mesh Terms:

KEYWORDS: Sequence Alignment

MESH TERMS: immunology

Chemical & Substance for Abstract: A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display. Information

Substance Name: Peptide Library

Registry Number: 0

Grant and Affiliation Information for A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display.

AFFILIATION: Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China.

Country: Japan

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MEDLINETA: J Biochem

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